Anti-human globulin (Coombs) reagent
Anti-Human Globulins are prepared from serum of immunized rabbits and from serum of a mouse hybridoma cell line and are used for the detection of in-vivo or in-vitro coating of red cells with immunoglobulin or complement.
They are used in the direct and indirect Coombs tests.
Anti-Human Globulins are available as a green or clear solution. Meanwhile the green solution is used most because it allows for a visual control of the reagent being added.
Anti-Human Globulin Mono-TypeŽ
This polyspecific Coombs reagent is a mixture of rabbit anti-IgG and mouse monoclonal anti-C3b and anti-C3d.
The potent anti-IgG part increases the resistance against neutralization of the Coombs reagent due to incomplete removal of human serum.
The use of monoclonal anti-C3b and anti-C3d allows for precise titer adjustment of each individual anti-complement and increases the sensitivity especially for the direct Coombs test. The high specificity is achieved through the complete absence of anti-C4 and anti-T.
Characteristics
- Optimal combination of IgG and complement antibodies
- Optimal concentration of anti-C3b and anti-C3d
- Free from anti-C4 and anti-T
- Available in clear and green solution
- Constant titer from lot to lot
- Also available in 50 ml volumes
Monospecific Anti-Human Globulins
For the evaluation of positive direct antiglobulin tests, a range of monospecific anti-human sera are available :
- Anti-IgG
- Anti-IgA
- Anti-IgM
- Anti-C3
- Anti-C3d
- Anti-C4