Sopachem
TransIT®-293 Transfection Reagent


The TransIT®-293 Transfection Reagent was specifically developed to achieve the highest transfection efficiency in HeK 293 cells. This ready-to-use reagent results in 75–85% transfection efficiency using EGFP as a reporter, with minimal cellular toxicity. Highest transfection efficiencies are achieved when transfections are performed in serum-containing media, with no media change. MIR 2700 provides sufficient reagent to perform up to 500 transfections in 35 mm dishes or 6-well plates.

To top

TransIT®-3T3 Transfection Kit


The TransIT®-3T3 Transfection Kit was specifically developed to achieve the highest transfection efficiency in NIH 3T3 cells. This kit, which consists of a TransIT®-3T3 Reagent and a 3T3 Authority Reagent, results in 55–65% transfection efficiency using EGFP as a reporter, with minimal cellular toxicity. Highest transfection efficiencies are achieved when transfections are performed in serum-containing media, with no media change. MIR 2180 provides sufficient amounts of both reagents to perform up to 500 transfections in 35 mm dishes or 6-well plates.

To top

TransIT®-CHO Transfection Kit


The TransIT®-CHO Transfection Kit was specifically developed to achieve the highest transfection efficiency in CHO-K1 cells. This kit, which consists of a TransIT®-CHO Reagent and a CHO Mojo Reagent, results in 50–60% transfection efficiency using EGFP as a reporter, with minimal cellular toxicity. Highest transfection efficiencies are achieved when transfections are performed in serum-containing media, with no media change. MIR 2170 provides sufficient amounts of both reagents to perform up to 500 transfections in 35 mm dishes or 6-well plates.

To top

TransIT®-COS Transfection Kit


The TransIT®-COS Transfection Kit was specifically developed to achieve the highest transfection efficiency in COS-1 and COS-7 cells. This kit, which consists of a TransIT®-COS Reagent and a COS Boss Reagent, results in 90–95% transfection efficiency using EGFP as the reporter, with minimal cellular toxicity. Highest transfection efficiencies are achieved when transfections are performed in serum containing media, with no media change. MIR 2190 provides sufficient amounts of both reagents to perform up to 500 transfections in 35 mm dishes or 6-well plates.

To top

TransIT-HeLaMONSTeR® Transfection Kit


The TransIT-HeLaMONSTeR® Transfection Kit was specifically developed to achieve the highest transfection efficiency in HeLa cells. This kit, which consists of a TransIT®-HeLa Reagent and MONSTER Reagent, results in 50–60% transfection efficiency using EGFP as a reporter, with minimal cellular toxicity. Highest transfection efficiencies are achieved when transfections are performed in serumcontaining media, with no media change. MIR 2900 provides sufficient amounts of both reagents to perform up to 500 transfections in 35 mm dishes or 6-well plates.

To top

TransIT®-Insecta Transfection Reagent


The TransIT®-Insecta Transfection Reagent was specifically developed to achieve the highest transfection efficiency in insect cell lines. This ready-to-use reagent results in 40–60% transfection efficiency in Sf9 cells, using beta-galactosidase as a reporter, with minimal cellular toxicity. Transfections can be performed with or without serum.

MIR 2200 provides sufficient reagent to perform up to 250 transfections in 35 mm dishes or 6-well plates.

To top

TransIT®-Jurkat Transfection Reagent


The TransIT®-Jurkat Transfection Reagent is a novel formulation optimized to provide superior transfection efficiency in Jurkat cells and other lymphoid-like cell lines. Jurkat cells (human T-lymphocyte, acute T-cell leukemia) have been inherently difficult to transfect. This reagent results in 10–25% transfection efficiency in Jurkat cells using EGFP as a reporter and FACS analysis. Highest transfection efficiencies are achieved when transfections are performed in serum-containing media, with no media change. MIR 2120 provides sufficient reagent to perform up to 500 transfections in 35 mm wells or 6-well plates.

To top

TransIT®-Keratinocyte Transfection Reagent


The TransIT®-Keratinocyte Transfection Reagent was specifically developed to achieve the highest transfection efficiency in keratinocytes. This ready-to-use reagent results in 20–30% transfection efficiency using EGFP as a reporter, with minimal cellular toxicity. Highest transfection efficiencies are achieved when transfections are performed in serum-containing media, with no media change. MIR 2800 provides sufficient reagent to perform up to 500 transfections in 35 mm dishes or 6-well plates.

To top

TransIT-Neural® Transfection Reagent


The TransIT-Neural® Transfection Reagent is a novel formulation specifically developed to achieve superior transfection efficiency in a wide variety of neural cell types while maintaining high cell viability. This ready-to-use reagent results in up to 75% efficiency using EGFP as a reporter plasmid. Highest efficiencies are achieved when transfections are performed in serum-containing media, with no media change. MIR 2140 provides sufficient reagent to perform up to 500 transfections in 12-well plates.

NEURAL CELL LINES SUCCESSFULLY TRANSFECTED By MIRUS BIO : C6, Daoy, DB-TRG-05MG, DI-TNC1, HCN-1A, Neuro-2a, PC-12, SK-N-MC, SVGp12.

To top

TransIT®-Prostate Transfection Kit


The TransIT®-Prostate Transfection Kit is optimized to provide superior transfection efficiency in prostate cells while maintaining high cell viability. This kit results in greater than 50% efficiency in prostate cell lines using EGFP as a reporter. Highest efficiencies are achieved when transfections are performed in serum-containing media, with no media change. MIR 2130 provides sufficient reagent to perform up to 500 transfections in 35 mm wells or 6-well plates.

PROSTATE CELL LINES SUCCESSFULLY TRANSFECTED By MIRUS BIO: DU 145, LNCaP, PC3.

To top

Documents section

 

Download catalogue (5.9 Mb)

Download protocols. You must be registered to access these documents. Please click here to register.

 

Request a sample


Click here to request a sample