Performa® Spin Columns feature a fully hydrated gel matrix that provides for a convenient and simple method to purify your DNA/RNA samples.  They effectively remove salts, amino acids, nucleotides, traces of solvents and other low molecular weight materials from nucleic acid fragments greater than 20 bases.

Benefits
Cleanup DNA fragments as large as 50kb
Up to 95% recovery depending on fragment size
Start to finish in only 5 minutes
Sample is eluted and ready for downstream applications

Applications
Purification of labeled DNA : eliminate unincorporated labeled nucleotides and probes
Desalting : removes up to 99.99% of salts and other small molecules
Alternative method to alcohol precipitation : 99% removal of trace chloroform and phenol, as well as DMSO
RNA PAGE purification : recover 80-90% of small RNAs from denaturing PAGE gels

The PurElute™ Bacterial Genomic Kit provides rapid isolation of genomic DNA from gram-positive and gram-negative bacteria utilizing just one simple protocol.  In only 30 minutes you can extract high-quality DNA, saving you time without sacrificing performance.  Specially formulated Advamax™ 2 Beads efficiently remove protein and other cellular contaminants from DNA. 

Benefits
DNA is suitable for even your most sensitive downstream applications
Consistently provides high-quality DNA
Only 30 minutes from start to finish
Average yield is 40 µg of DNA
Process in single tube format or multi-well formats

Applications
PCR
Restriction digests
Direct bacterial genome sequencing
Southern blotting

The PurElute™ Miniprep Kit was developed for rapid isolation and purification of high-copy and low-copy plasmids as well as cosmids.  The kit utilizes a modified alkaline lysis protocol and can be used in a centrifuge or a vacuum manifold in less than 20 minutes processing time.  An innovative spin basket design ensures enough volume under the drip director to avoid the need for a second spin during binding.  Purified DNA can be used for most molecular biology downstream applications.

Benefits
High purity DNA : Endonuclease-free
High yield : Up to 20 µg
Rapid protocol : Less than 20 minutes
Excellent DNA recovery : 85-95%
Flexible format : Centrifuge or vacuum
User-friendly : Simple spin-column format
PurBlue : Visualize complete lysis to maximize DNA yield

Applications
Restriction Digestion
PCR
Cloning
Sequencing
Ligation and Transformation

PurElute™ IEX Plasmid Kits present a novel ion-exchange membrane for purification of superior quality endotoxin-free plasmid DNA.  Membrane porosity was optimized to provide high interior surface area accessible for DNA binding and high flow rate for easy sample processing.  This method is simpler and more efficient than conventional gravity flow columns.  PurElute™ IEX Plasmid Kits have an exceedingly high binding capacity, excellent selectivity and recovery efficiency.  Purified plasmid DNA is free of impurities that may interfere with demanding downstream applications.

Benefits
Superior quality DNA : Suitable for mammalian cell transfections and other sensitive downstream applications
High yield : Binding capacity greater than conventional gravity flow columns
Quick protocol : Significantly faster than conventional methods
Simple spin column format : No waiting for gravity flow columns
Broad applications : Purify plasmids of all sizes, cosmids, fosmids, and BACs Ready to use : No pre-wetting or equilibration step
High purity DNA : Maxiprep provides endotoxin-free DNA
PurBlue : Visualize complete lysis to maximze DNA yield

Applications
Enzymatic modifications
Transfection of sensitive eukaryotic cells
Gene therapy research
In-vitro transcription/translation
Plasmid-mediated gene silencing

The PurElute™ GX DNA Gel Extraction & Cleanup Kit is designed for the easy extraction and purification of dsDNA from solutions or agarose gel in TAE or TBE buffers. A Binding Buffer containing chaotropic salt and reinforced buffer dissolves agarose gel and facilitates the capture of DNA fragments >70 bp in a spin column. The column is washed and the purified DNA is eluted in 15-50µl of Elution Buffer or water.

Benefits
High purity DNA
Eliminate primers, primer-dimers, dNTPs, salts and DNA labels
Recovery >75-95%, depending on fragment size and elution volume
Rapid protocol : less than 20 minutes
Convenient processing options : centrifuge or vacuum
User-friendly : simple spin column format
Purifies almost any fragment size : 70bp – 10kb
Binding capacity up to 5µg of DNA

Applications
Further Amplification
Sequencing
Restriction Digestion
Cloning

Quick-Precip™ Solution is a proprietary, biologically inert, carrier used for rapid precipitation of DNA, RNA, and oligomers >16 bases.  Precipitation with >95% efficiency is accomplished in 2-3 minutes at room temperature.  Quick-Precip™ Solution is not affected by the presence of additives frequently found in enzymatic reactions or residual organic solvents used for extraction of proteins.  Because Quick-Precip™ Solution is biologically inert, precipitated DNA or RNA can be used for hybridization, cloning, preparation of libraries and PCR.