ELISA kits & reagents

ELISA (enzyme-linked immunosorbent assay) is a commonly used application for the detection of antigens in a microwell format. The assay may be performed using the direct, indirect, or sandwich method and requires the use of a substrate that produces soluble end products. The results can be qualitative or quantitative, where specific levels of product are read in a spectrophotometer or luminometer. In a properly optimized assay, the intensity of color, or light, generated is directly proportional to the amount of reactant present.

KPL provides a variety of reagents designed for all levels of users, from the beginner to the experienced researcher.

Protein Detector ELISA kits provide a convenient starting point for the development of ELISA protocols. Each kit includes a comprehensive instruction manual for developing enzyme immunoassay procedures as well as pretested components including blocking, coating and wash solutions along with affinity-purified conjugates. Users only need to provide primary antibody and plates to run an ELISA.
KPL offers Protein Detector ELISA kits with anti-mouse, anti-rabbit or anti-human secondary antibodies labeled with alkaline phosphatase (AP) or horseradish peroxidase (HRP).

ELISA substrates (chromogenic and chemiluminescent) that can be used with alkaline phosphatase or horseradish peroxidase are also available. Chromogenic substrates produce a colored end product, which can be detected and measured visually or with the use of a spectrophotometer. Chemiluminescent substrates produce light, which can be measured using a luminometer or permanently recorded through exposure to X-ray film. Chemiluminescent substrates provide higher sensitivity than chromogenic substrates.