KAPA Probe Fast qPCR kits
KAPA PROBE FAST qPCR Kits are designed for high throughput, fast-cycling, real-time PCR using sequence-specific fluorogenic probes. These kits are compatible with all fluorogenic probe-based technologies, including hydrolysis probes (e.g. TaqManŽ) and displacement probes (e.g. molecular beacons).
The KAPA PROBE FAST qPCR Master Mix (2X) is a ready-to-use cocktail containing all components except primers, probe(s) and template for fast cycling probe-based real-time PCR. The 2X Master Mix contains KAPA Taq HotStart DNA polymerase, KAPA PROBE FAST qPCR Buffer, dNTPs, MgCl2 and stabilizers.
KAPA Taq HotStart DNA Polymerase is an antibody-mediated hot start formulation of KAPA Taq DNA polymerase. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.