KAPA SYBR Fast qPCR kits
KAPA SYBR® FAST qPCR Master Mix is designed for high performance real-time PCR. The kit contains a novel DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry.
The reaction efficiency of qPCR is critical for accurate and reliable gene quantification. Wild type Taq DNA polymerase is often inhibited by real-time PCR reaction conditions, leading to significant loss in specificity and sensitivity of the assay. The KAPA SYBR® DNA Polymerase has been engineered to perform optimally in stringent qPCR reaction conditions, exhibiting dramatic improvements in signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity, and sensitivity. The novel DNA polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC and AT-rich templates.
High speed is achieved through the increased processivity of the engineered KAPA SYBR® DNA Polymerase and the short initial denaturation time required for the activation of the proprietary antibody-mediated hot start.
Product Applications
KAPA SYBR® qPCR Kits are ideally suited for:
- Gene expression analysis
- Microarray validation
- Low copy gene detection
- Gene knockdown validation