KAPAHiFi™ DNA Polymerase

KAPAHiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends.

Processivity is defined as the number of nucleotides incorporated by the polymerase per binding event, and is a major determinant of extension speed. In contrast to fusion technologies that attach DNA binding proteins to Pfu-like polymerases to improve processivity, KAPAHiFi DNA Polymerase has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPAHiFi results in significant improvements in yield, speed, target length, and the ability to amplify difficult templates.

Typical proofreading polymerases require extension times of 1 - 2 minutes per kilobase. KAPAHiFi DNA Polymerase requires extension times of just 30 seconds per kilobase, providing the potential to save up to 75% of total reaction time. KAPAHiFi also possesses a strong 3’-5’ exonuclease-dependent proofreading activity resulting in superior accuracy.

KAPAHiFi DNA Polymerase kits include two buffers for optimal performance with difficult templates. Both buffers contain magnesium chloride allowing for convenient setup and robustness.

Product Applications

KAPAHiFi DNA Polymerase is designed for high fidelity PCR where amplified product is cloned for use in downstream applications such as:

• Site-directed mutagenesis
• Sequencing
• Protein expression