KAPALongRange DNA Polymerase

The KAPALongRange PCR system is a blend of Taq DNA polymerase and a modified archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long range and/or sensitive PCR. The KAPALongRange system polymerizes DNA from a primer annealed to a DNA template in the presence of deoxyribonucleotide triphosphates. Both enzymes possess 5’→3’ polymerase activity, but only Taq possesses double strand dependent 5’→3’ exonuclease activity and only the Type B polymerase possesses 3’→5’ exonuclease (proofreading) activity.

KAPALongRange possesses higher fidelity than Taq polymerase. KAPAFidelity is recommended for shorter targets where higher fidelity is required.

All KAPALongRange kits are supplied with 5x KAPALongRange Buffer and 250 µl KAPA dNTPs (10 mM each). This buffer has been optimized for demanding applications that require long range and high sensitivity. Magnesium chloride solution is supplied separately to accommodate PCR optimization.

Product Applications

The KAPALongRange PCR system is suited for:

• PCR amplification of long targets and/or PCR using low concentrations of template DNA
• Standard short- and mid-range PCR amplification
• Production of PCR products to be used for ligation into 3’-T-overhang cloning vectors