PCR purification : Quickstep2
QuickStep™2 PCR Purification Systems offer a fast and simple method to highly purify your PCR products. Purified dsDNA passes through the system, while contaminants are captured by the SOPE™ Resin and Performa® Gel Filtration matrix. Patented SOPE™ (Solid-phase Oligo Protein Elimination) Resin binds and efficiently removes primers, ssDNA, enzymes and other proteins while the Performa® Gel Filtration matrix efficiently removes >99.9% of salts, buffers, dNTPs and other low molecular weight materials.
Benefits
Quick 2-spin protocol : start to finish in only 5 minutes
Excellent recoveries : 75%-99% recovery depending on fragment size
High purity : eliminate primers, ssDNA, proteins, salts, dNTPs and DNA labels
Purifies almost any fragment size : 100 bp-48.5 kb
Minimal DNA manipulation : does not bind your DNA. Just add your sample and spin once
Flexible formats : single, 96- and 384-well formats
Applications
Further amplification
Sequencing
Restriction digestion
Cloning