The Pippin Prep is a system that separates DNA using agarose gel electrophoresis, and then electroelutes DNA fractions according user-input fragment size ranges. This virtually eliminates the labor required for manual prep gels, and provides a higher quality sample for NGS. The system includes a disposable five-channel pre-cast gel cassette, and a computerized instrument which combines an electrophoresis platform with a fluorescencebased DNA detection unit. During operation, software uses the optical system and a DNA size ladder to determine the correct timing to extract a fragment size range directly from agarose. Extractions are undertaken by switching the electrophoretic path into an intersecting channel which collects the eluted fraction in a buffer-filled chamber. At the end of a run, DNA fractions are removed using a standard pipette.