Empore™ 96-Well Solid Phase Extraction Plates are designed for high throughput solid phase extraction (SPE). 96 samples can be processed within a standard 8 row by 12 column microtiter plate format. One disk plate can replace four separate runs on a conventional SPE manifold handling 24 individual cartridges per run. The 96 well format is ideal for sample preparation prior to LC/ MS/MS or other high throughput analytical techniques.
The plate is molded from a polypropylene resin. An Empore™ extraction disk is secured in place at the bottom of each well with a sealing ring. A proprietary prefilter is placed above the Empore disk. This prefilter aids in preventing particulates and macromolecules from reaching the underlying membrane and improves the flow of biological samples, such as serum and plasma, through the plate.
The prefilter is composed of polypropylene microfiber layers of graded densities. Three different densities are used, with the coarsest one on top and the finest at the bottom. The top two microfiber layers are individual layers of material. The third microfiber layer, having the smallest effective pore size, is on the bottom of the prefilter and contains five individual layers of material. A porous polypropylene support membrane comprises the final layer.

Product Characteristics

Each well of the Empore™ 96-Well Solid Phase Extraction Plate has an effective membrane diameter of 5.5 mm. Effective diameter is the diameter of membrane available for use during sample processing. This diameter is smaller than the actual well diameter due to the dimensions of the rings that seal the membrane and prefilter into place. The reservoir volume of the standard plate is 1.2 ml and the volume of the deep well plate is 2.5 ml. If sample or reagent volumes exceed the volume of the well, multiple aliquots of solution may be used. Standard density (SD) membranes are composed of chromatographic particles commonly referred to as from 30 - 60 µ in size. The standard density membrane has been optimized for improved flow rates for samples processed in most bioanalytical applications.

Product Selection

Empore™ extraction disks are available in a variety of sorbent chemistries to compliment most analytical applications :

C8
C18
MPC (Mixed Phase Cation)
UR (Universal Resin)
Filter Plate