Transfection kit for biotherapeutic protein production
TransIT-PRO® Transfection Kit
Ideal for Biotherapeutic Protein Production
* Achieve high protein yield in suspension CHO and 293 cells
* Compatible with multiple CHO media formulations
* Reproducible protein expression with minimal optimization
Reach critical stages faster. Decrease time to produce usable protein by maximizing target protein yields through transient transfection. The TransIT-PRO® Transfection Kit uses animal origin free components designed for high and reproducible protein yield in suspension CHO and 293 derived cells. Since it is compatible with varied media formulations, the same media can be used for both transient and stable expression. The TransIT-PRO outperforms linear PEI in protein yield, while providing a cost-effective alternative to FreeStyle™ MAX.
Achieve High Antibody Titers Using the TransIT-PRO® Transfection Kit in Suspension CHO Cells. Human IgG1 was produced by transient transfection using TransIT-PRO and PRO Boost Reagent (1:1:1), 25 kDa linear PEI (6:1) or FreeStyle™ MAX (1:1) transfection reagents according to the manufacturers or published protocol (reagent:DNA ratio). Transfections were performed using 1 µg plasmid DNA per milliliter of culture and 0.5 x 106 cells/ml at the time of transfection. FreeStyle™ CHO-S cells were cultured in 20 ml of FreeStyle™ CHO Expression medium in 125 ml shake flasks. Day 3, 5 and 7 supernatants were clarified and analyzed using a human IgG-Fc sandwich ELISA. Error bars represent the standard deviation of triplicate technical replicates, 25kDa linear PEI is duplicate technical replicates.