Western blotting kits

When analyzing proteins immobilized on membrane, you’ll get consistent, high quality results with KPL’s Protein Detector™ Western Blot Kits. Choose from a broad line of kits and stand alone reagents for either chemiluminescent or colorimetric methods of detecting and quantifying specific antigens in a complex protein mixture. The cornerstone of Protein Detector systems is KPL's line of highly sensitive liquid substrates. You have a choice among multiple chemiluminescent and chromogenic solutions for peroxidase and phosphatase that provide varied levels of sensitivity.






The newest addition, LumiGLO Reserve™ Chemiluminescent Substrate, enables you to detect even lower amounts of protein than ever before. As a result of this option, you are now offered greater flexibility in selecting an assay that meets your changing research needs. These substrates are combined with unique blocking and wash solutions and highly specific secondary antibodies to create comprehensive, fully optimized kits for Western blotting. KPL has designed each of these kits to deliver the maximum signal to noise ratio available.
With Protein Detector, you will achieve clean, clear results blot after blot.







Signal linearity and signal-to-noise ratio comparison of advanced chemiluminescent substrates. Mouse IgG (250 pg to 3.91 pg) was transferred to nitrocellulose. Blots were detected under identical conditions with a 1:10,000 dilution of HRP-Goat-anti-Mouse IgG (except ECL Advance, 1:100,000) and exposed to film for 10 minutes. Densitometry was then performed using the Syngene GeneGenius. LumiGLO Reserve demonstrates superior signal linearity over a larger dynamic range and the greatest comparative signal-to-noise.