Automated DNA Size Selection Systems
These systems automate the collection of specific DNA size ranges. By replacing manual gel excision, they eliminate cross-contamination and provide unparalleled reproducibility in fragment sizing.
Manual gel cutting is prone to human error and inconsistent yields. Sage Science systems use integrated software and optical detection to collect DNA fractions automatically into buffer, ensuring that every library is sized with mathematical precision.
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Choose the Right Pippin System for Your Lab
Pippin Prep
The industry standard for routine NGS size selection between 50bp and 1.5kb.
BluePippin
Expanded range capability using pulsed-field electrophoresis for fragments up to 50kb.
PippinHT
High-capacity platform processing up to 24 samples per run for large-scale sequencing projects.
Automated NGS Library Construction
Precision Sizing for Short-Read Workflows
As a benchmark for automated DNA size selection, the Pippin Prep is recommended by leading platform providers for producing tightly sized insert libraries. It replaces manual gel purification, eliminating operator-dependent variability and cross-contamination. The system typically processes up to five samples per cassette in 30 to 60 minutes, ensuring high-quality DNA for applications like RNA-seq and ChIP-seq.
HMW DNA and Protein Selection
Pulsed-Field Power for Long-Read Applications
The BluePippin expands on standard Pippin capabilities by incorporating pulsed-field electrophoresis. This allows for the precise collection of high molecular weight (HMW) DNA up to 50kb, essential for long-read sequencing technologies. Beyond DNA, the system can also utilize SDS-agarose gel cassettes to collect specific protein sizes, making it a versatile tool for both genomics and proteomics research.
High-Throughput DNA Size Selection
Efficient Automation for Large-Scale Sequencing
The PippinHT scales up the proven sizing technology of the Pippin line to accommodate 24 samples per run. Designed for busy sequencing hubs and core facilities, it offers half the run time and lower sample costs compared to lower-throughput models. The system maintains high reproducibility and recovery rates while utilizing an SBS-footprint cassette compatible with robotic workflows.
- Precision DNA fragment collection
- Automated electrophoresis workflow
- High reproducibility for NGS
- Optimized for long-read sequencing
- Bench-top sizing technology
- Precision DNA fragment collection
- Automated electrophoresis workflow
- High reproducibility for NGS
- Optimized for long-read sequencing
- Bench-top sizing technology
Common Questions Regarding Automated Size Selection
Essential information for integrating Pippin technology into laboratory workflows.
Automation ensures precise and narrow fragment distributions, which maximizes the efficiency of sequencer flow cells by preventing the loading of unwanted artifacts like adapter-dimers or overly long fragments.
No, internal markers and software protocols are instrument-specific. While the technology is similar, users must select cassette kits specifically validated for their respective instrument model.
Yes, unused lanes can be reserved for future use. For the PippinHT, lane pairs can be resealed with PCR tape and run at a later time, provided it is within the cassette's expiration date.
Users generally observe an intrinsic yield of 50% to 80% of the DNA within the targeted size range. Yield can be optimized by broadening the selection range or using specific elution rinsing techniques.
Gel cassettes should be stored at room temperature, while liquid reagents such as markers and buffers should be refrigerated at 4°C. Most components have a shelf life of approximately 18 months.
DNA collected from dye-free cassettes is typically ready for immediate use in NGS workflows. Samples from cassettes containing ethidium bromide will require a standard cleanup step before proceeding.