Azure Biosystems Sapphire Scanner
The Sapphire FL is a next-generation laser scanning platform designed to adapt to the evolving needs of modern research labs. Whether performing routine Western blots or complex live-animal imaging, the system delivers exceptional sensitivity and a broad linear dynamic range. By utilizing a modular design with user-interchangeable optical components, it eliminates the need for multiple single-application instruments and you will be able to address evolving research questions easily. The sapphire boasts the widest possible wavelength range supporting wavelengths from 375nm up to 900nm.
| Feature | Sapphire FL | Sapphire FL+ |
| Scanning Area | 25 x 25 cm | 46 x 25 cm |
| Resolution | 5 μm – 1000 μm | 5 μm – 1000 μm |
| Detectors | APD, PMT, sCMOS | APD, PMT, sCMOS |
| Z-Plane Range | -1.0 to +6 mm | -1.0 to +6 mm |
| Wavelengths | 375 nm – 900 nm | 375 nm – 900 nm |
| Dynamic Range | Up to 24-bit | Up to 24-bit |
| In Vivo Imaging | Integrated Anesthesia Ports | Not Available |
| Article Number | Description |
| IS4000 | Sapphire FL Biomolecular Imager |
| IS4100 | Sapphire FL+ Biomolecular Imager |
| IS4007 | Sapphire FL Chemiluminescence Module |
| IS4101 | Sapphire FL+ Chemiluminescence Module |
| IS1015 | Sapphire Eraser (for phosphor screens) |
| AC1132 | Azure Spot (Imaging Software) |
| AC1078 | Azure Spot Pro (Imaging Software) |
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Applications
One System, Every Sample
Blots & Gels
High-sensitivity detection for multiplex Westerns, 2D DIGE, and DNA/RNA gels.
Slide Imaging
Automated imaging for up to 20 standard slides at 5-micron resolution.
Phosphor Imaging
Compatible with all major storage phosphor screens for radioisotope detection.
Optical Versatility
Unlimited Laser and Filter Wavelength Combinations
The Sapphire FL features a unique, patent-pending design of interchangeable and customizable laser and filter modules, enabling a virtually infinite number of spectral combinations. Supporting a broad range of excitation and emission wavelengths as well as phosphor imaging, it is the only biomolecular scanner in its class to reach into the UV spectrum. This allows for the multiplexing of UV dyes (such as DAPI and Hoechst) with NIR dyes using the 375 Standard Optical Module.
Precision Imaging in Action
Experience the Versatility of the Sapphire FL
Gain a comprehensive understanding of how the Sapphire FL series streamlines complex imaging workflows through its advanced laser scanning technology. Our product specialists are available to provide personalized demonstrations, showcasing the full range of applications and modular configurations possible with this platform to meet your specific research requirements.
Technical & Operational Overview
Frequently asked questions regarding the modular architecture and application capabilities of the Sapphire FL and FL+ platforms.
The Sapphire FL series features user-interchangeable laser and filter modules. This patent-pending design allows laboratories to adapt the system to new dyes or changing experimental requirements in under two minutes without requiring a technician, ensuring the instrument remains future-proof as research evolves.
Both models offer identical scanning performance, 5-micron resolution, and modularity. The Sapphire FL+ features an expanded scanning bed of 46 x 25 cm (compared to 25 x 25 cm on the standard FL), making it ideal for high-throughput workflows and large-format samples like oversized phosphor screens or multiple multi-well plates.
Yes. The instruments feature a software-controlled adjustable Z-plane with a 7 mm focal range (from -1 mm to +6 mm). This allows for optimized imaging of various formats, including thin membranes, microscope slides, 96-well plates, and even small organisms with up to 4 cm of clearance.
The systems utilize Extended Dynamic Range (EDR) technology, providing 24-bit data depth. This allows for the simultaneous detection and quantification of high-abundance and low-abundance proteins (femtogram sensitivity) in a single scan without signal saturation.
Yes. While the standard configuration excels at fluorescence and phosphor imaging, an optional Chemiluminescence Module can be added to provide high-resolution quantitative imaging of chemiluminescent blots and visible color markers.