BrightBox™ NGS Library Quantitation
Library quantitation is often the most tedious step in the Next Generation Sequencing (NGS) workflow. Traditional qPCR methods are accurate but slow and prone to pipetting errors, while fluorometric methods (like Qubit) are fast but often inaccurate because they measure non-sequenceable DNA.
BrightBox™ bridges this gap. It is a novel, ultra-fast assay that accurately quantitates any library with P5 and P7 adapters. By eliminating the need for serial dilutions and long cycling times, BrightBox delivers reliable molarity data in a fraction of the time—allowing you to load your flow cells with confidence.
Method: qPCR / Fluorescence
Target: Illumina P5/P7 Adapter Sequences
Time to Result: ~5 Minutes
Instrumentation: Any qPCR System (FAM/ROX)
Standards: Pre-diluted (Included)
Dynamic Range: Linear across standard NGS library concentrations
| Article Number | Description | Pack Size |
| 50100 | BrightBox™ Assay Mix & Controls (100 reactions) | 1 Kit |
| 50500 | BrightBox™ Assay Mix & Controls (500 reactions) | 1 Kit |
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Why Choose BrightBox?
The BrightBox Advantage
Rapid Results
Complete your quantitation in 5 minutes. The optimized protocol requires only a short incubation and read step, compared to 2.5 hours for standard qPCR.
Ultimate Accuracy
Targets only sequenceable molecules (with proper adapters), ensuring your pool loading concentration matches the actual cluster density.
Zero Prep
Comes with pre-diluted standards and a ready-to-use Assay Mix. No complex serial dilutions means fewer manual errors and less hands-on time.
Smart Design
Streamlined Workflow
BrightBox utilizes a unique qPCR-based chemistry that correlates fluorescence directly to library molarity without the need for extensive thermal cycling.
- Universal Compatibility: Works on any qPCR instrument or plate reader capable of reading FAM and ROX channels.
- Linear & Reproducible: High linearity across a wide dynamic range, ensuring consistent pooling.
- Cost Effective: Reduces waste and "re-runs" caused by unequal library loading.
- The BrightBox Workflow:
- Aliquot Assay Mix.
- Add Libraries & Pre-diluted Standards.
- Incubate & Read (5 mins).