Cielo qPCR Cycler
The Azure Cielo qPCR cycler is engineered to provide precise amplification and high-speed quantification for diverse biological samples. With a unique 16-well simultaneous scanning head with fiber optic technology, the system delivers uniform excitation and and reads emission fast and uniform across the entire plate. This design specifically eliminates the need for passive reference dyes like ROX, allowing for more flexible channel use and reduced reagent costs.
| Feature | Cielo 3 | Cielo 6 |
| Detection Channels | 3 | 6 |
| Multiplexing | Up to 3 targets | Up to 6 targets |
| Sample Capacity | 96-well plate/tubes/strips | 96-well plate/tubes/strips |
| Reaction Volume | 1–50 µL | 1–50 µL |
| Temp. Uniformity | ±0.2°C | ±0.2°C |
| Max Ramp Rate | 6.0°C/sec | 6.0°C/sec |
| Excitation Range | 450–730 nm | 450–730 nm |
| Article Number | Description |
| AIQ030 | Azure Cielo 3 Real-Time PCR System, 96 well, 3 channel |
| AIQ060 | Azure Cielo 6 Real-Time PCR System, 96 well, 6 channel |
| AIQ100 | Software for plate setup, data analysis and remote device control. Unlimited installations, Windows PC compatible. |
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Automated Notifications
Stay informed without manual monitoring. The system sends end-of-run notifications and completed data files directly to your inbox via Wi-Fi or Ethernet, allowing for efficient time management.
Application Flexibility
Available in 3-channel or 6-channel configurations, the Cielo accommodates a diverse range of qPCR chemistries and multiplexing requirements. The open-platform design ensures compatibility with standard 96-well plates and various master mixes.
Intelligent Workflow
Streamline assay setup with intuitive, user-friendly software. Features such as a large integrated touchscreen and customizable protocols reduce manual programming time and minimize the risk of setup errors.
Robust and Reliable Data
Achieve superior reproducibility through a high-performance thermal block and a fixed-path fiber optic system. This robust design ensures consistent well-to-well results and long-term instrument stability for sensitive quantification.
Experience the Azure Cielo Workflow
See the Azure Cielo in action. This brief overview demonstrates the system’s high-performance thermal block and intuitive integrated software, highlighting how the 96-well scanning technology delivers precision and speed for your real-time PCR assays.
Data Visualization
Individualized Amplification Curve Analysis
The Azure Cielo Manager software provides complete control over data visualization. Users can view comprehensive datasets simultaneously or isolate specific wells and targets for detailed inspection. The interface supports seamless toggling between linear and log views, with customizable color-coding by Sample ID or target to ensure clear data differentiation.
High-Resolution Imaging
Unrivaled Sensitivity with 100,000 Data Points per Well
The Azure Cielo sets a new standard for optical resolution by capturing approximately 100,000 pixels per well. This dense data acquisition provides a more accurate and sensitive representation of fluorescent intensities compared to competitive systems that acquire only a single pixel per well. In performance benchmarking, this increased resolution allowed the Cielo to detect single-copy amplification in 92% of plate wells, significantly outperforming the 80% detection rate of standard industry systems.
Validate Your Research
See the Cielo in Your Lab
Discover how the Azure Cielo can enhance your laboratory's qPCR performance. Schedule a demo with our product specialists to explore the system's high-sensitivity optics and intuitive workflow first-hand.
Technical & Integration Insights
Find detailed answers to common technical inquiries regarding the Azure Cielo’s optical technology, system integration, and performance benchmarks for high-throughput laboratory environments.
The system utilizes a high-performance thermal block with ±0.2°C uniformity and a fixed-path fiber optic system. This hardware stability, combined with the removal of optical path variation, ensures that results are highly reproducible between runs and different instrument units, making it ideal for multi-site B2B research operations.
No. The Azure Cielo is an open-format platform compatible with standard 96-well plates and tubes. Its advanced optics eliminate the need for ROX, allowing you to use a wider range of high-efficiency master mixes while reducing the cost per reaction.
The Cielo 6 allows for the detection of up to six targets in a single well. This significantly increases throughput and reduces sample consumption, which is critical for complex screening, pathogen detection, and gene expression studies where sample volume is limited.
Yes. The system is designed with connectivity in mind, featuring Wi-Fi, Ethernet, and USB ports. The Azure Cielo Manager software is built to facilitate easy data export and remote monitoring, supporting the integration requirements of modern, digitally-connected laboratories.
By capturing 100,000 data points per well, the Cielo offers superior sensitivity for detecting low-copy-number targets. This high-resolution imaging reduces background noise and improves the reliability of results for sensitive applications like rare mutation detection or low-viral-load quantification.