FUJIFILM Anti Iba1, Rabbit (for Immunocytochemistry)
This affinity-purified rabbit polyclonal antibody specifically targets Ionized Calcium-Binding Adapter Molecule 1 (Iba1/AIF1), a canonical marker for cells of macrophage and microglial lineage. Formulated as a liquid reagent, each production lot undergoes rigorous sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis and functional validation to guarantee high purity and minimal lot-to-lot variability for downstream research applications.
| Technical Parameter | Specification Value |
| Target Antigen | Ionized Calcium-Binding Adapter molecule 1 (Iba1) |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Physical State / Appearance | Liquid |
| Validated Applications | Immunocytochemistry (ICC), Immunohistochemistry on frozen sections (IHC(F)) |
| Quality Control Criteria | Electrophoresis test (SDS-PAGE) to pass test |
| Application Grade | for Immunochemistry |
| Article Number | Description | Pack Size |
| 019-19741 | Anti Iba1, Rabbit (for Immunocytochemistry) | 50 µg |
| 013-27691 | Anti Iba1, Rabbit (for Immunocytochemistry on Parafin Embedded Tissues) | 50 µg |
| Conjugated versions | ||
| Article Number | Description | Pack Size |
| 016-26461 | Anti Iba1, Rabbit, Biotin-conjugated | 100uL |
| 015-28011 | Anti Iba1, Rabbit, SPICA Dye™ 568-conjugated | 100uL |
| 012-28401 | Anti Iba1, Rabbit, SPICA Dye™ 594-conjugated | 100uL |
| 013-26471 | Anti Iba1, Rabbit, Red Fluorochrome (635)-conjugated | 100uL |
| Looking for a conjugated version? Contact us for availability and technical advice. | ||
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Core Product Capabilities
Process-Level Definition
Specially selected and refined to allow complete visualization of cell structures, providing clear resolution of delicate microglial processes and branches under fluorescence microscopy.
Broad Target Species Utility
Demonstrates extensive target reactivity across mouse, rat, and human tissues, maximizing experimental utility within common neurodegenerative research models.
Economical Dilution Ratios
Highly concentrated formulation allows efficient laboratory working dilutions ranging from 1:500 to 1:1,000, supporting long-term project longevity.
Biological Markers & Mechanisms
Characterizing Microglial Responses in Central Nervous System Research
Ionized calcium-binding adapter molecule 1 (Iba1) is a carbohydrate-binding protein localized inside microglial populations within the central nervous system. During neurodegenerative processes, such as the accumulation of amyloid-beta plaques in model systems, these immune cells undergo phenotypic changes and migrate around protein aggregates. Utilizing this antibody allows for the precise, selective staining required to track microglial morphology and density across varied experimental setups.
Reference Methodologies
Immunohistochemistry Protocol for Microglia
- Perfuse and fix experimental models using 4% paraformaldehyde-phosphate buffer. Note: Inadequate or missing perfusion fixation directly leads to suboptimal staining results.
- Submerge tissues in sucrose for cryoprotection and prepare standard frozen matrix blocks.
- Cut sections to an optimal thickness of 20 µm to 50 µm utilizing a freezing microtome.
- Wash tissue slices using a 0.3% Triton X-100 in PBS solution three times for 5 minutes per cycle.
- Incubate in blocking buffer (1% BSA, 0.3% Triton X-100 in PBS) for 2 hours at ambient room temperature.
- Note: If high background noise occurs, increase blocking exposure times or evaluate alternative blocking matrices (such as 1% BSA with 0.3% Tween-20 in PBS, or 3% normal serum matching the secondary host species).
- Dilute the primary antibody in blocking buffer at a recommended working ratio of 1:500 to 1:1,000.
- Apply the solution and incubate overnight at a controlled temperature of 4°C. Note: While incubation variables vary by assay environment, successful microglial labeling has been completed in alternative samples like rat cerebellum within 2 hours.
- Rinse the sections three times for 5 minutes each utilizing 0.3% Triton X-100 in PBS.
- Dilute a fluorescently labeled anti-rabbit IgG secondary conjugate in blocking buffer at a working ratio of 1:500 to 1:1,000.
- Incubate the sections for 1 to 2 hours at ambient room temperature. Note: If excessive background interference appears, shorten the secondary antibody exposure time.
- Perform a final sequence of three washes for 5 minutes each in 0.3% Triton X-100 in PBS. Note: Increase the total number of washing cycles if background signal persists.
- Secure the treated slices onto glass slides using high-quality mounting media and capture images via fluorescence or confocal microscopy.
If staining intensity remains weak due to sample fixation conditions, perform an explicit retrieval sequence immediately after sectioning:
- Heat samples in Citrate buffer (pH 6.0) for 9 minutes at 90°C.
- Alternately, heat samples in TE buffer (pH 9.0) for 9 minutes at 90°C.
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Peer-Reviewed Research & Literature
Official Product Citations
Explore the dedicated database of registered studies and literature utilizing the FUJIFILM Wako Anti-Iba1 antibody.
PubMed Central Search
Access real-time peer-reviewed open-access papers and research articles tracking this specific antibody clone via PMC.
- EXPERTLY VALIDATED FOR IMMUNOCYTOCHEMISTRY
- STABLE LOT-TO-LOT REPRODUCIBILITY VIA SDS-PAGE TESTING
- MOLECULAR MARKER OF CHOICE FOR MACROPHAGE AND MICROGLIAL RETRIVAL PROJECTS
- FOR LABORATORY RESEARCH USE ONLY
- EXPERTLY VALIDATED FOR IMMUNOCYTOCHEMISTRY
- STABLE LOT-TO-LOT REPRODUCIBILITY VIA SDS-PAGE TESTING
- MOLECULAR MARKER OF CHOICE FOR MACROPHAGE AND MICROGLIAL RETRIVAL PROJECTS
- FOR LABORATORY RESEARCH USE ONLY
Technical Support & Protocols
Essential technical parameters and optimization tips for managing immunochemical assays.
The antibody is raised against a synthetic peptide corresponding directly to the conserved C-terminal sequence of the Iba1 protein.
No. This catalog entry is optimized and validated exclusively for immunocytochemistry and frozen tissue sections. For blotting applications, please select a specialized option.
Extend the initial blocking incubation time or update the formulation. Recommended blocking options include PBS supplemented with 1% BSA and 0.3% TritonX-100, or adding 3% normal serum obtained from the secondary antibody host organism.
For optical results and clean visualization of complex cell structures, it is highly recommended to prepare fresh frozen blocks sectioned at a thickness of 20 µm to 50 µm.
When utilizing a standard protocol with a 1:1,000 dilution ratio and applying a standard 200 µL working solution per slide, a single vial provides enough material for approximately 500 tests.