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Mirus Bio Broad Spectrum Transfection Reagents & Solutions

High-performance nucleic acid delivery solutions for transient and stable expression in mammalian cell types.
Mirus Bio Cell culture & transfection

Mirus Bio broad-spectrum transfection reagents enable robust delivery of plasmid DNA, mRNA, and siRNA across diverse mammalian cell lines, including hard-to-transfect and primary cells. Designed for minimal toxicity and serum compatibility, these systems eliminate the need for culture media changes, streamlining laboratory workflows and supporting high-throughput screening.

Ordering Information

Catalog NumberProduct DescriptionPack Contents / Scale
MIR 2304TransIT-LT1 Transfection Reagent1 x 0.4 mL
MIR 2300TransIT-LT1 Transfection Reagent1 x 1.0 mL
MIR 2305TransIT-LT1 Transfection Reagent5 x 1.0 mL
MIR 2306TransIT-LT1 Transfection Reagent10 x 1.0 mL
Catalog NumberProduct DescriptionPack Contents / Scale
MIR 5404TransIT-2020 Transfection Reagent1 x 0.4 mL
MIR 5400TransIT-2020 Transfection Reagent1 x 1.0 mL
MIR 5405TransIT-2020 Transfection Reagent5 x 1.0 mL
MIR 5406TransIT-2020 Transfection Reagent10 x 1.0 mL

Catalog Number

Product Description

Pack Contents / Scale

MIR 6003TransIT-X2 Dynamic Delivery System1 x 0.3 mL
MIR 6004TransIT-X2 Dynamic Delivery System1 x 0.75 mL
MIR 6000TransIT-X2 Dynamic Delivery System1 x 1.5 mL
MIR 6005TransIT-X2 Dynamic Delivery System5 x 1.5 mL
MIR 6006TransIT-X2 Dynamic Delivery System

10 x 1.0 mL  / 10 x 1.5 mL

Catalog Number

Product Description

Pack Contents / Scale

MIR 2225TransIT-mRNA Transfection Kit1 x 0.4 mL TransIT-mRNA Reagent, 1 x 0.4 mL mRNA Boost Reagent
MIR 2250TransIT-mRNA Transfection Kit1 x 1.0 mL TransIT-mRNA Reagent, 1 x 1.0 mL mRNA Boost Reagent
MIR 2255TransIT-mRNA Transfection Kit5 x 1.0 mL TransIT-mRNA Reagent, 5 x 1.0 mL mRNA Boost Reagent
MIR 2256TransIT-mRNA Transfection Kit10 x 1.0 mL TransIT-mRNA Reagent, 10 x 1.0 mL mRNA Boost Reagent
Ingenio Electroporation Solutions & Systems

Catalog Number

Product Description

Pack Contents / Scale

MIR 50111Ingenio Electroporation Solution1 x 6.25 mL (25 rxns in 0.4 cm cuvettes / 62.5 rxns in 0.2 cm)
MIR 50114Ingenio Electroporation Solution1 x 12.5 mL (50 rxns in 0.4 cm cuvettes / 125 rxns in 0.2 cm)
MIR 50117Ingenio Electroporation Solution2 x 12.5 mL (100 rxns in 0.4 cm cuvettes / 250 rxns in 0.2 cm)
MIR 50112Ingenio Electroporation Kit for Amaxa1 x 6.25 mL Solution, 25 x 0.2 cm Cuvettes, 25 Cell Droppers
MIR 50115Ingenio Electroporation Kit for Amaxa1 x 12.5 mL Solution, 50 x 0.2 cm Cuvettes, 50 Cell Droppers
MIR 50118Ingenio Electroporation Kit for Amaxa2 x 12.5 mL Solution, 100 x 0.2 cm Cuvettes, 100 Cell Droppers
MIR 50113Ingenio Electroporation Kit for Open Systems1 x 6.25 mL Solution, 25 x 0.4 cm Cuvettes, 25 Cell Droppers
MIR 50116Ingenio Electroporation Kit for Open Systems1 x 12.5 mL Solution, 50 x 0.4 cm Cuvettes, 50 Cell Droppers
MIR 50119Ingenio Electroporation Kit for Open Systems2 x 12.5 mL Solution, 100 x 0.4 cm Cuvettes, 100 Cell Droppers
MIR 50108Ingenio 0.2 cm Electroporation Cuvettes50 Pack Cuvettes (for EZporator / Lonza Nucleofector)
MIR 50109Ingenio 0.4 cm Electroporation Cuvettes50 Pack Cuvettes (for EZporator / Harvard-BTX / Bio-Rad)
MIR 50110Ingenio Cell Droppers50 Pack Cell Droppers
MIR 50100Ingenio EZporator Electroporation System1 Electroporation Unit

Performance Optimization & Application Guidelines

  • DNA Delivery: TransIT-LT1, TransIT-2020, and TransIT-X2 provide efficient delivery of plasmid DNA across standard and hard-to-transfect cell types. Optimal results are achieved using high-purity, endotoxin-free plasmid DNA with an A260/280 ratio between 1.8 and 2.0.

  • siRNA & Co-Transfection: TransIT-X2 enables single siRNA knockdown as well as simultaneous delivery of plasmid DNA and siRNA with low cellular toxicity.

  • mRNA Delivery: TransIT-mRNA Transfection Kit utilizes a dual-component system (TransIT-mRNA Reagent + mRNA Boost Reagent) designed to transfect 2-10 kb mRNA directly into the cytoplasm, bypassing nuclear transcription barriers.

  • Electroporation Compatibility: Ingenio Electroporation Solution functions universally with open and closed systems (including Bio-Rad Gene Pulser, Harvard-BTX, and Lonza-Amaxa Nucleofector) across exponential decay and square waveform programs.

  • Transfection Reagents
  • Electroporation Solutions
  • DNA & RNA Delivery
  • Serum Compatible
  • Animal-Origin Free
  • Plasmid DNA
  • Hard-to-Transfect Lines
  • Transfection Reagents
  • Electroporation Solutions
  • DNA & RNA Delivery
  • Serum Compatible
  • Animal-Origin Free
  • Plasmid DNA
  • Hard-to-Transfect Lines

Frequently Asked Questions

Procurement, regulatory compliance, and support details for professional facility management.

Analytical certificates, Certificates of Analysis (CoA), and detailed Safety Data Sheets (SDS) are provided for every production lot to meet institutional and regulatory standards.

Multi-pack quantities (up to 10 x 1.0 mL or 10 x 1.5 mL configurations) are listed standard, and custom bulk sizing can be arranged upon technical evaluation.

Reagents requiring -20 deg C or 4 deg C storage are shipped under strict temperature-controlled packaging to maintain guaranteed shelf life.

Formulations such as TransIT-2020 are completely synthetic and animal-origin free, mitigating regulatory concerns regarding biological cross-contamination.

Reagent Agent, an online selection engine, provides cell-type-specific performance metrics, citation lists, and starting optimization protocols.

All transfection reagents and solutions are covered by a full 1-year product performance guarantee when handled and stored according to manufacturer instructions.

Technical Documentation

TransIT-LT1 Transfection Reagent User Protocol

Detailed instructions, culture vessel scaling ratios, and troubleshooting guidelines for high-efficiency plasmid DNA transfection.
Download

TransIT-2020 Transfection Reagent User Protocol

Operational protocol for animal-origin free plasmid DNA delivery in mammalian cells.
Download

TransIT-X2 Dynamic Delivery System User Protocol

Complete workflow steps for single DNA, siRNA, and co-transfection experiments using non-liposomal polymeric technology.
Download

TransIT-mRNA Transfection Kit User Protocol

Comprehensive procedure for cytoplasmic mRNA transfection utilizing dual-reagent complexing.
Download

Ingenio Electroporation Kits and Solution User Protocol

Program guidelines, pulse conditions, and cell-density recommendations for universal electroporation instruments.
Download

Ingenio Electroporation Solution Safety Data Sheet (SDS)

Official safety, handling, storage, and regulatory compliance data for Ingenio Electroporation Solution.
Download