Mirus Bio Cell and Gene Therapy Virus Production
Mirus Bio provides high-performance transfection reagents and kits optimized for Adeno-Associated Virus (AAV) and Lentivirus (LV) vector manufacturing. Engineered to support transition from discovery to clinical manufacturing, these reagents deliver robust viral titers in both adherent and suspension cell culture platforms.
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- Scalable Viral Vector Manufacturing
- GMP-Grade Transfection Reagents
- High Titer AAV & Lentivirus Production
- Advanced Transfection Systems
- Scalable Viral Vector Manufacturing
- GMP-Grade Transfection Reagents
- High Titer AAV & Lentivirus Production
- Advanced Transfection Systems
Ordering Information
| Catalog Number | Product Description | Pack Contents / Scale |
MIR 73750 | TransIT® AAViator™ Transfection System | 2 x 1.5 mL TransIT AAViator, 1 x 1.5 mL RevIT Enhancer (1 L culture) |
MIR 73745 | TransIT® AAViator™ Transfection System | 7 x 30 mL TransIT AAViator, 10 x 1.5 mL RevIT Enhancer (15 L culture) |
MIR 73720 | TransIT® AAViator™ Transfection System | 1 x 450 mL TransIT AAViator, 1 x 200 mL RevIT Enhancer (200 L culture) |
| Catalog Number | Product Description | Pack Contents / Scale |
| MIR 6703 | TransIT-VirusGEN® Transfection Reagent | 0.3 mL |
| MIR 6700 | TransIT-VirusGEN® Transfection Reagent | 0.75 mL |
| MIR 6704 | TransIT-VirusGEN® Transfection Reagent | 1.5 mL |
| MIR 6705 | TransIT-VirusGEN® Transfection Reagent | 5 x 1.5 mL |
| MIR 6706 | TransIT-VirusGEN® Transfection Reagent | 10 x 1.5 mL |
| MIR 6710 | TransIT-VirusGEN® Transfection Reagent | 30 mL |
| MIR 6715 | TransIT-VirusGEN® Transfection Reagent | 150 mL |
| Catalog Number | Product Description | Pack Contents / Scale |
| MIR 8000 | RevIT™ AAV Enhancer | 1.5 mL (for 1 L of cell culture) |
| MIR 8006 | RevIT™ AAV Enhancer | 10 x 1.5 mL |
| MIR 8080 | RevIT™ AAV Enhancer | 75 mL |
| MIR 8200 | RevIT™ AAV Enhancer | 200 mL (for 200 L of cell culture) |
| Catalog Number | Product Description | Pack Contents / Scale |
| MIR 8007 | VirusGEN® AAV Transfection Kit with RevIT AAV Enhancer | 2 x 1.5 mL TransIT-VirusGEN, 2 x 150 µL Complex Stabilizer, 1 x 1.5 mL RevIT Enhancer |
| MIR 8008 | VirusGEN® AAV Transfection Kit with RevIT AAV Enhancer | 1 x 30 mL TransIT-VirusGEN, 2 x 1.5 mL Complex Stabilizer, 10 x 1.5 mL RevIT Enhancer |
| Catalog Number | Product Description | Pack Contents / Scale |
| MIR 6815-GMP | VirusGEN® GMP AAV Transfection Kit | 1 x 150 mL TransIT-VirusGEN GMP, 5 x 1 L Complex Formation Solution & Enhancer |
| MIR 6825-GMP | VirusGEN® GMP LV Transfection Kit | 1 x 150 mL TransIT-VirusGEN GMP, 5 x 7 L LV Enhancer, 5 x 7 L Complex Formation Solution |
| MIR 6845-GMP | TransIT-VirusGEN® GMP Transfection Reagent | 1 x 150 mL |
| MIR 8000-GMP | RevIT™ GMP AAV Enhancer | |
| MIR 6816-GMP | VirusGEN® GMP Transfection Complex Stabilizer | 15 mL (for 50 L of cell culture) |
Product Categories
Explore Vector Production Systems
TransIT-AAViator Transfection System
High-yield transfection system designed specifically for suspension AAV production workflows.
VirusGEN Transfection Reagents
Versatile, high-efficiency transfection reagent for scalable lentivirus and AAV generation.
RevIT AAV Titer Enhancer
Performance booster providing 2-4 fold yield increases in AAV vector production.
VirusGEN AAV Transfection Kits
All-in-one kit solutions combining transfection reagent and titer enhancers for optimized workflows.
GMP Products
Fully qualified, sterile, endotoxin-tested reagents and kits for clinical viral manufacturing.
Technical Documentation
VirusGEN® GMP AAV Transfection Kit Protocol
VirusGEN® GMP LV Transfection Kit Protocol
RevIT™ AAV Enhancer Quick Reference Protocol
TransIT-VirusGEN® GMP Reagent Protocol
VirusGEN® AAV Transfection Kit with RevIT Protocols
Frequently Asked Questions
Common technical and operational questions regarding vector manufacturing and reagent scaling.
Yes, TransIT-VirusGEN® reagents and kits are engineered for high-efficiency transfection in both adherent, serum-containing cultures and serum-free suspension HEK 293 platforms. Specific complex formation guidelines and optimization protocols are available for each culture format.
RevIT™ AAV Enhancer works synergistically with TransIT-VirusGEN® transfection reagents to boost AAV vector production yields by 2- to 4-fold in suspension HEK 293 workflows. It can be added directly to transfection complexes or added directly to the cell culture.
TransIT-VirusGEN® GMP reagents and associated kits undergo stringent release testing, including assays for appearance, formulation identity, sterility, bacterial endotoxins, mycoplasma, pH, and osmolality to ensure compliance for clinical vector manufacturing.
No, there is no need to perform a complete media change post-transfection. Transfection complexes can remain in the culture medium throughout the full incubation period without compromising cell viability or viral titers.
A complex formation incubation time of 15–30 minutes at room temperature is recommended as a standard starting point. For large-scale bioreactor workflows, complex formation times can be evaluated between 5 and 60 minutes depending on the specific vector construct and vessel dynamics.
TransIT-VirusGEN® reagents and RevIT™ Enhancers should be stored frozen at -10°C to -30°C. Complex formation solutions and LV enhancers should be stored at 2°C to 10°C. All components must be warmed to room temperature and gently mixed or vortexed prior to complex formation.