Mirus Bio Protein Production & Expression Systems
The Mirus Bio CHOgro® High Yield Expression System is an optimized platform specifically engineered to enhance transient protein expression in suspension CHO cell cultures. By combining high-density cell growth with a high-efficiency transfection protocol and a specialized titer enhancer, this system enables researchers to achieve significantly higher protein yields (up to 10-fold) compared to standard methods. The entire workflow is animal-origin-free and designed for quick implementation without the need for complex licensing agreements.
Parameter | Technical Details |
| Target Cell Lines | Suspension CHO (e.g., FreeStyle™ CHO-S, ExpiCHO-S™), HEK 293 derivatives |
| Formulation | Chemically defined, hydrolysate-free, animal-origin free |
| Downstream Compatibility | Eliminates post-transfection culture media changes |
| TransIT-PRO® Storage | -20°C |
| CHOgro® Titer Enhancer Storage | 2°C to 10°C, protected from light |
| Expression Medium Storage | 4°C, protected from light |
| Poloxamer 188 Storage | Room temperature |
| L-Glutamine Storage | -10°C to -30°C |
| Product Name | Product No. | Package Size / Volume |
| CHOgro® High Yield Expression System | MIR 6270 | 1 Kit (2x 1000 ml Medium, 1 ml TransIT-PRO®, 20 ml Enhancer, Solution, Supplements) |
| TransIT-PRO® Transfection Reagent Kit | MIR 5740 | 1 x 1.0 ml |
| TransIT-PRO® Transfection Reagent Kit | MIR 5750 | 1 x 10 ml |
| CHOgro® Expression Medium | MIR 6200 | 1 x 1000 ml |
| CHOgro® Complex Formation Solution | MIR 6210 | 1 x 100 ml |
| CHOgro® Titer Enhancer | MIR 6220 | 1 x 20 ml |
| Poloxamer 188, 10% Solution | MIR 6230 | 1 x 100 ml |
| L-Glutamine, 200 mM Solution | 59202C-100mL | 1 x 100 ml |
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Transient Protein Expression
Streamlined High-Titer Expression in Suspension CHO Cells
The CHOgro® High Yield Expression System is an integrated, chemically defined platform engineered for high-density transient protein production in suspension CHO cell lines. Incorporating advanced lipid-polymer transfection technology and specialized titer enhancement formulations, the system eliminates the need for culture media changes while maximizing protein yield in batch shaker flask or bioreactor operations.
Product Range
Integrated Protein Production Solutions
CHOgro® High Yield Expression System
Full system providing media, L-Glutamine, Poloxamer 188, complex solution, TransIT-PRO® reagent, and CHOgro® Titer Enhancer for optimal CHO production.
TransIT-PRO® Transfection Reagent
Animal-origin free transfection reagent developed via empirical screening of lipid/polymer libraries for suspension CHO and HEK 293 cells.
Media & Reagents Components
Standalone CHOgro® Expression Medium, Complex Formation Solution, Poloxamer 188, and specialized enhancers for flexible scaling.
Technical Guidelines
Operational Considerations & Optimization
CHOgro Expression Medium requires supplementation with 4 mM L-Glutamine and 0.3% Poloxamer 188 prior to use. Suspension CHO lines typically adapt directly or via step-wise sequential adaptation (e.g., 75/25 to 100% ratios). Passaging should be performed to maintain cell densities between 2.5 x 10^5 and 1 x 10^7 cells/ml.
For optimal results, passaging 18-24 hours prior to transfection ensures active cell division. Target seeding density is 4 x 10^6 cells/ml at the time of transfection. Complex formation should occur in CHOgro Complex Formation Solution with a 5-minute incubation period at room temperature. Post-transfection, an immediate shift to mild hypothermal conditions (32 degrees C) enhances specific productivity and extends culture longevity.
- Scalable Viral Vector Manufacturing
- High-Efficiency AAV & Lentivirus Yields
- GMP-Compliant Reagents & Systems
- Streamlined Scale-Up from Bench to Bioreactor
- Scalable Viral Vector Manufacturing
- High-Efficiency AAV & Lentivirus Yields
- GMP-Compliant Reagents & Systems
- Streamlined Scale-Up from Bench to Bioreactor
Frequently Asked Questions
Key technical and operational details regarding vector production reagents and scaling.
Yes, TransIT-VirusGEN® reagents and kits are formulated for high-yield transfection in both adherent, serum-containing cultures and serum-free suspension HEK 293 systems. Dedicated complex formation guidelines are provided for each culture format
RevIT™ AAV Enhancer works synergistically with TransIT-VirusGEN® transfection reagents to increase AAV vector yields 2- to 4-fold in suspension HEK 293 workflows. It can be added during complex formation or introduced directly into the culture.
TransIT-VirusGEN® GMP reagents and kits undergo quality control testing for appearance, formulation identity, sterility, bacterial endotoxins, mycoplasma, pH, and osmolality to support clinical manufacturing requirements.
No media change is necessary post-transfection. Transfection complexes remain in the culture medium throughout the full incubation period without compromising cell health or viral titers.
A complex formation incubation of 15–30 minutes at room temperature serves as a standard starting point. For large-scale vessel processing, complex formation times can be evaluated between 5 and 60 minutes based on specific construct dynamics.
TransIT-VirusGEN® reagents and RevIT™ Enhancers must be stored frozen at -10°C to -30°C. Complex formation solutions and LV enhancers are stored at 2°C to 10°C. Reagents should be warmed to room temperature and mixed gently before complex preparation.