PCR Purification Products
Successful downstream analysis—whether sequencing, cloning, or genotyping—depends on clean DNA templates. EdgeBio offers a versatile suite of PCR purification technologies to remove primers, nucleotides (dNTPs), salts, and enzymes from your amplification products.
Choose from the rapid 5-minute QuickStep™ 2 spin protocol, the high-throughput ExcelaPure™ ultrafiltration plates, or the zero-loss EnzSAP™ enzymatic cleanup to perfectly match your laboratory’s workflow and automation needs.
Optimized for Your Workflow
Speed & Purity
QuickStep™ 2 is the premier choice, delivering sequencing-ready DNA in minutes without ethanol precipitation.
(5 minute protocol)
Minimal Handling
EnzSAP™ allows you to treat samples directly in the PCR tube or plate—perfect for automated liquid handlers.
(non-spin workflow)
Large Volumes
ExcelaPure™ handles up to 300 µL per well, making it ideal for pooling reactions or cleaning up dilute samples.
(high-volume recovery)
High-Efficiency Dye Terminator Removal
Performa DTR Gel Filtration Systems
These spin columns are designed to provide rapid and effective removal of salt and dye terminators from DNA sequencing reactions. Utilizing a specialized gel filtration matrix, the system ensures high recovery rates of purified DNA while maintaining the integrity of long-read sequences.
| Article Number | Description | Format | Qty/Pack |
| 73328 | Performa® Spin Columns | Spin Columns | 36 Columns |
| 13266 | Performa® Spin Columns | Spin Columns | 36 Columns |
Rapid PCR Purification
QuickStep 2 PCR Cleanup System
The QuickStep 2 system offers a streamlined approach to PCR purification by utilizing a dual-action filtration and proprietary resin technology. This process eliminates primers, dNTPs, and salts in a single five-minute centrifugation step, yielding highly purified DNA suitable for immediate downstream applications such as sequencing and cloning.
| Article Number | Description | Format | Qty/Pack |
| 33617 | QuickStep™ 2 Cartridge PCR Purification Kit | Spin Cartridges | 36 Preps |
| 92159 | QuickStep™ 2 Cartridge PCR Purification Kit | Spin Cartridges | 108 Preps |
| 61303 | QuickStep™ 2 96-Well PCR Purification Kit | 96-Well Plates | 2 Plates |
| 97670 | QuickStep™ 2 96-Well PCR Purification Kit | 96-Well Plates | 10 Plates |
| 05086 | QuickStep™ 2 96-Well PCR Purification Kit | 96-Well Plates | 50 Plates |
| 39231 | QuickStep™ 2 384-Well PCR Purification Kit | 384-Well Plates | 2 Plates |
| 89815 | QuickStep™ 2 384-Well PCR Purification Kit | 384-Well Plates | 10 Plates |
| 72412 | QuickStep™ 2 SOPE™ Resin | Solution | 3.5 mL |
High-Throughput DNA Purification
ExcelaPure 96-Well Cleanup Plates
Optimized for automated workflows, ExcelaPure plates provide consistent results for high-volume dye terminator removal. The 96-well format is compatible with standard robotic systems and thermal cyclers, ensuring uniform sample treatment and high-quality capillary electrophoresis results across all samples.
| Article Number | Description | Format | Qty/Pack |
| 39614 | ExcelaPure™ 96-Well UF PCR Purification Kit | 96-Well Plate | 1 Plate |
| 84900 | ExcelaPure™ 96-Well UF PCR Purification Kit | 96-Well Plate | 10 Plates |
| 36181 | ExcelaPure™ 96-Well UF Plates (No Receivers) | 96-Well Plate | 10 Plates |
| 95674 | ExcelaPure™ 96-Well UF Plates (No Receivers) | 96-Well Plate | 50 Plates |
Enzymatic PCR Product Cleanup
EnzSAPE Reagent System
EnzSAPE provides a convenient enzymatic method for purifying PCR products prior to sequencing. By employing a combination of Exonuclease I and Shrimp Alkaline Phosphatase, this reagent effectively degrades excess primers and dephosphorylates remaining dNTPs without the need for columns or centrifugation steps.
| Article Number | Description | Format | Qty/Pack |
| EnzSAP-500 | EnzSAP™ PCR Clean-up Reagent | Tube | 500 Rxns |
| EnzSAP-2000 | EnzSAP™ PCR Clean-up Reagent | Tube | 2,000 Rxns |
- High-efficiency primer and dNTP removal
- Up to 95% DNA recovery across broad fragment sizes
- Streamlined 5-minute centrifugation protocols
- Automated high-throughput 96-well and 384-well formats
- Enzyme-based purification without columns or centrifugation
- High-efficiency primer and dNTP removal
- Up to 95% DNA recovery across broad fragment sizes
- Streamlined 5-minute centrifugation protocols
- Automated high-throughput 96-well and 384-well formats
- Enzyme-based purification without columns or centrifugation
Technical Support & Frequently Asked Questions
Detailed information on optimizing your purification workflows for Sanger sequencing and other genomic applications.
Depending on the specific kit and fragment size, researchers can expect highly reproducible recovery rates of up to 95%.
The protocols are designed for maximum efficiency; for instance, the Performa and QuickStep 2 systems utilize a two-spin process that can be completed in approximately five minutes.
Yes, several solutions like ExcelaPure and QuickStep 2 are available in 96-well and 384-well formats, specifically optimized for integration into robotic liquid handling systems.
These systems efficiently eliminate unincorporated primers, dNTPs, salts, enzymes, and other low-molecular-weight materials that can interfere with downstream analysis.
Performa Spin Columns are capable of cleaning up DNA fragments as large as 50 kb while maintaining high recovery and sequence integrity.
The EnzSAP reagent system provides an entirely enzymatic approach that removes primers and dephosphorylates dNTPs without the need for columns or centrifugation steps.