PCRBIO Hotstart Taq DNA Polymerase & Mixes
PCRBIO HS Taq DNA Polymerase is a high-performance solution engineered for laboratory applications requiring absolute precision and the detection of low-copy number targets. By utilizing a sophisticated antibody-mediated hot start mechanism, the enzyme remains sequestered and completely inactive during reaction setup at room temperature, effectively eliminating the formation of primer dimers and non-specific products. This robust system features advanced buffer chemistry pre-optimized for consistent results across a broad range of challenging templates, including GC-rich (up to 71%) and AT-rich sequences, without the need for additional enhancers. Its high inhibitor tolerance makes it particularly suited for direct PCR from crude samples such as whole blood, urine, and bacterial colonies, while its availability in high-concentration, lyophilisation-compatible formats (250 U/µL) provides B2B partners and diagnostic kit manufacturers with the flexibility needed for automated liquid handling and customized assay development.
| Feature | Specification |
| Hot Start Method | Antibody-mediated |
| Activation Temperature | 95°C |
| Inactivation Threshold | Below 65°C |
| Maximum Amplicon Length | Up to 6kb |
| Template Compatibility | GC/AT-rich, mammalian genomic DNA, blood, urine |
| Purification | 12-step strategy (physical, chemical, enzymatic) |
| Product Code | Description | Pack Size | Presentation |
| PB10.21-02 | PCRBIO HS Taq DNA Polymerase | 250 Units | [1 x 0.05ml 5 units/µL] & [2 x 1mL buffer] |
| PB10.21-10 | PCRBIO HS Taq DNA Polymerase | 1000 Units | [4 x 0.05ml 5 units/µL] & [8 x 1mL buffer] |
| PB10.21-50 | PCRBIO HS Taq DNA Polymerase | 5000 Units | [20 x 0.05ml 5 units/µL] & [40 x 1mL buffer] |
| PB10.22-02 | PCRBIO HS Taq Mix | 200 Reactions | 5 x 1mL |
| PB10.22-10 | PCRBIO HS Taq Mix | 1000 Reactions | 5 x (5 x 1mL) |
| PB10.23-02 | PCRBIO HS Taq Mix Red | 200 Reactions | 5 x 1mL |
| PB10.23-10 | PCRBIO HS Taq Mix Red | 1000 Reactions | 5 x (5 x 1mL) |
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Efficient GC-Rich Amplification
PCRBIO HS Taq Mix Red is engineered to overcome the common challenges of complex templates. By utilizing advanced antibody-mediated hot start technology and a specialized buffer system, it delivers high-yield amplification of GC-rich fragments (up to 71% GC) and AT-rich sequences without the need for additional enhancers or optimization. This ensures reliable results across a broad range of genomic targets.
Superior Sensitivity
Effectively detect low abundance targets with unrivalled sensitivity down to 5pg of template DNA.
Robust Performance
Engineered for high success rates on difficult GC and AT-rich templates without requiring specialized buffers.
Streamlined Workflow
Available as a 2x ready mix with an optional red dye for direct gel loading and tracking.
Performance & Integration Insights
Detailed technical information regarding the integration of PCRBIO HS Taq into professional laboratory workflows, including automation compatibility, specialized assay development, and complex template handling.
The antibody-mediated mechanism ensures the polymerase remains completely inactive at room temperature. This prevents the formation of non-specific products and primer dimers during high-throughput setup, resulting in superior sensitivity and consistent performance across varying batch sizes.
Yes. The stability of the hot start technology allows for extended reaction setup at room temperature without loss of specificity. Its robust buffer chemistry is designed to maintain consistency in automated, high-throughput workflows.
For partners developing proprietary kits, we offer HS Taq DNA Polymerase in a high-concentration (250 U/µL) format. This version is specifically optimized for lyophilisation and provides maximum flexibility when volume is limited in complex assay formulations.
PCRBIO HS Taq is engineered with high inhibitor tolerance, making it suitable for direct PCR from unprocessed samples such as blood, urine, and bacterial colonies. This reduces the need for extensive DNA purification steps, accelerating the time-to-result for screening applications.
The advanced buffer system is specifically pre-optimized to handle difficult templates. It facilitates efficient denaturation and primer annealing on sequences with up to 71% GC content, eliminating the need for separate additives like DMSO or specialized high-GC buffers.
