PCRBIO Rapid Extract Kits
The PCRBIO Rapid Extract PCR Kit combines a rapid lysis system with a high-performance hot start Taq master mix. This integrated approach eliminates time-consuming DNA purification steps, providing PCR-ready DNA in just 15 minutes. The system is engineered to maximize speed and yield while minimizing contamination risks by performing extraction in a single tube.
| Feature | Specification |
| Extraction Time | 15 minutes |
| Format | Single-tube, Column-free |
| Polymerase | Antibody-mediated hot start Taq |
| Integrated Dye | Red mix for direct gel loading |
| Downstream | Endpoint PCR & qPCR |
| Product Code | Description | Pack Size |
| PB10.24-08 | PCRBIO Rapid Extract PCR Kit | 80 Reactions |
| PB10.24-40 | PCRBIO Rapid Extract PCR Kit | 400 Reactions |
| PB15.11-08 | PCRBIO Rapid Extract Lysis Kit | 80 Reactions |
| PB15.11-24 | PCRBIO Rapid Extract Lysis Kit | 240 Reactions |
| PB10.23-02 | PCRBIO HS Taq Mix Red | 200 Reactions |
| PB10.23-10 | PCRBIO HS Taq Mix Red | 1000 Reactions |
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Dual-Action Hot Start
Antibody-mediated inhibition prevents non-specific amplification and primer dimers at room temperature.
Direct Gel Loading
The HS Taq Mix Red includes a non-inhibitory tracking dye, allowing samples to be loaded directly onto agarose gels after PCR.
qPCR Compatible
Supernatants are fully compatible with both endpoint PCR and real-time qPCR platforms.
Technical Support & Frequently Asked Questions
Get guidance on optimizing rapid DNA extraction workflows and ensuring high-specificity amplification for clinical and research applications.
By utilizing a single-tube, column-free extraction process, the system reduces total preparation time to just 15 minutes. This eliminates the need for multiple centrifugation and wash steps, allowing laboratories to process larger sample batches with significantly reduced manual handling time and lower plastic waste.
The kit is optimized for a wide range of challenging biological materials, including mouse tail clips, ear punches, animal tissues, hair follicles, mammalian blood, buccal swabs, and FFPE samples. Its robust buffer system is designed to release high-quality DNA even from tough, solid tissues.
Yes. While the kit includes a high-performance hot start Taq for endpoint PCR, the extracted supernatant is fully compatible with both real-time qPCR and endpoint PCR platforms. For those using existing internal qPCR protocols, the Lysis-only variant provides the same rapid extraction efficiency without the polymerase component.
The entire extraction takes place in a single reaction tube. By avoiding the transfer of material between columns and collection tubes, the risk of aerosol contamination and sample loss—critical when working with limited or precious starting material—is virtually eliminated.
All PCRBIO Rapid Extract Kits are manufactured under a strict ISO 13485 certified Quality Management System. This ensures that every lot meets high standards for performance consistency, traceability, and reliability required for commercial diagnostic development and industrial-scale research.
Following the 15-minute protocol and debris pelleting, the resulting DNA supernatant can be used immediately or stored at -20 degrees. This stability allows for flexible scheduling in high-volume testing environments where extraction and amplification may occur in different shifts.
The chemistry is highly amenable to scale. Because the protocol relies on simple heat incubation and a single centrifugation step rather than complex vacuum or pressure-driven column steps, it is easily adapted for use with automated liquid handling systems and thermal blocks in 96-well formats.
