qPCRBIO Clara® Probe Real time PCR Mixes

The 4x concentration provides greater flexibility in assay design by allowing for a larger volume of template DNA or RNA. This is particularly beneficial when working with highly dilute samples or when adding multiple primer/probe sets in complex multiplex reactions, ensuring superior sensitivity without compromising reaction volume.

Yes. The mix is engineered with a high-performance, antibody-mediated hot start DNA polymerase that allows for rapid activation and high efficiency. It is fully optimized for both standard and fast cycling conditions across all real-time PCR instruments.

AquaPlex utilizes a unique passive reference dye that fluoresces in the Cy5 (red) channel. By moving the reference signal out of the ROX channel, it frees up that spectrum for an additional target probe. This reduces signal crosstalk and improves the reliability of results in highly multiplexed assays where the Cy5 channel is traditionally prone to lower signal-to-noise ratios.

Every production lot of Clara Probe Mix undergoes rigorous quality control protocols to ensure high sensitivity, low background signal, and consistent $C_q$ values. Our manufacturing processes are designed to meet the stringent requirements of diagnostic kit developers and commercial partners who require reliable longitudinal performance and a secure, high-quality supply chain.

For RNA targets, we offer the Clara Probe 1-Step Mix (including AquaPlex versions), which contains a modified UltraScript® Reverse Transcriptase and an advanced RNase inhibitor in a single-tube format. This allows for seamless, high-sensitivity RT-qPCR without the need for a separate cDNA synthesis step.

The Clara Probe Purple Mix contains a non-inhibitory purple dye that provides clear visual confirmation of mastermix and sample addition. This feature is a critical quality control step in manual high-throughput pipetting, significantly reducing the risk of human error and plate re-runs without affecting the fluorescence of the probes.

Yes. Antibody-mediated hot start provides a more "instant" activation during the initial denaturation step compared to chemical methods. This ensures maximum polymerase activity from the first cycle and superior suppression of non-specific amplification and primer-dimers at room temperature, which is essential for precise quantification of low-copy targets.

Absolutely. We provide Clara Probe Mixes in various scales, from 200-reaction bottles up to 50 mL bulk presentations (10,000+ reactions). We specialize in supporting B2B clients with scalable supply chains and can discuss custom packaging or concentrations to fit specific diagnostic kit requirements.