qPCRBIO SyGreen® Chameleon Kits
The qPCRBIO SyGreen Chameleon Kit range incorporates a dual-color tracking system to assist visual verification during real-time PCR setup. Combining a blue master mix with a yellow SampleTrack Buffer, the solution undergoes an immediate visual color shift to green upon sample introduction, reducing pipetting errors and blank wells in high-throughput protocols. Utilizing non-inhibitory intercalating dye technology and antibody-mediated hot-start polymerases, these kits support fast cycling conditions across diverse passive reference instruments.
| Parameter | Description |
| Detection Method | Non-inhibitory intercalating dye (SyGreen) |
| Tracking System | Dual-color visual shift (Blue mix + Yellow buffer = Green reaction) |
| Hot Start Mechanism | Antibody-mediated hot start DNA polymerase |
| Cycling Speeds | Standard and ultra-fast cycling (down to 2s denaturation, 2s annealing/extension) |
| Applications | Dye-based qPCR, relative quantification, absolute quantification, 2-step RT-qPCR |
| Passive Reference Options | Lo-ROX, Hi-ROX, No-ROX, Separate-ROX |
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How it works
It isn't rocket science: Blue + Yellow = Green
Setting up high-density qPCR plates can easily lead to tracking errors and costly carryover contamination. The qPCRBIO SyGreen Chameleon Kit transforms reaction setup into a foolproof visual workflow—delivering instant loading confirmation, universal instrument compatibility, and advanced contamination control before your run even starts.
The mastermix contains a vibrant blue tracking dye. This creates a clear visual baseline in your wells while actively preventing carryover contamination from previous PCR reactions. This mix is also available with integrated UNG/dUTP technology for labs repetively amplifying the same targets.
A non-interfering yellow buffer added directly to your DNA/RNA samples. Designed for universal compatibility, it maintains fast, highly sensitive quantification performance across standard qPCR instrument formats without affecting reaction kinetics.
Watch your mastermix immediately turn from blue to green as yellow sample is added. This instant visual color shift provides absolute certainty that every single well is correctly loaded—eliminating missed wells, double-dosing, and pipetting mistakes.
Visual Tracking qPCR Mix
qPCRBIO SyGreen Chameleon Kit
The qPCRBIO SyGreen Chameleon Kit includes the 2x qPCRBIO SyGreen Blue Mix alongside the 100x yellow SampleTrack Buffer. Mixing the yellow sample buffer into the blue reaction chemistry causes a clear transition to green, ensuring reliable tracking of sample addition across multi-well plates.
| Product Code | Description | Pack Size | Presentation |
PB20.81-03 | qPCRBIO SyGreen Chameleon Kit Lo-ROX | 300 x 20 µL rxns | 3 x 1 mL mix, 1 x 150 µL buffer |
PB20.81-12 | qPCRBIO SyGreen Chameleon Kit Lo-ROX | 1200 x 20 µL rxns | 12 x 1 mL mix, 4 x 150 µL buffer |
PB20.81-50 | qPCRBIO SyGreen Chameleon Kit Lo-ROX | 5000 x 20 µL rxns | 1 x 50 mL mix, 2 x 1.25 mL buffer |
PB20.81-51 | qPCRBIO SyGreen Chameleon Kit Lo-ROX | 5000 x 20 µL rxns | 50 x 1 mL mix, 2 x 1.25 mL buffer |
| Product Code | Description | Pack Size | Presentation |
PB20.82-03 | qPCRBIO SyGreen Chameleon Kit Hi-ROX | 300 x 20 µL rxns | 3 x 1 mL mix, 1 x 150 µL buffer |
PB20.82-12 | qPCRBIO SyGreen Chameleon Kit Hi-ROX | 1200 x 20 µL rxns | 12 x 1 mL mix, 4 x 150 µL buffer |
PB20.82-50 | qPCRBIO SyGreen Chameleon Kit Hi-ROX | 5000 x 20 µL rxns | 1 x 50 mL mix, 2 x 1.25 mL buffer |
PB20.82-51 | qPCRBIO SyGreen Chameleon Kit Hi-ROX | 5000 x 20 µL rxns | 50 x 1 mL mix, 2 x 1.25 mL buffer |
| Product Code | Description | Pack Size | Presentation |
PB20.83-03 | qPCRBIO SyGreen Chameleon Kit No-ROX | 300 x 20 µL rxns | 3 x 1 mL mix, 1 x 150 µL buffer |
PB20.83-12 | qPCRBIO SyGreen Chameleon Kit No-ROX | 1200 x 20 µL rxns | 12 x 1 mL mix, 4 x 150 µL buffer |
PB20.83-50 | qPCRBIO SyGreen Chameleon Kit No-ROX | 5000 x 20 µL rxns | 1 x 50 mL mix, 2 x 1.25 mL buffer |
PB20.83-51 | qPCRBIO SyGreen Chameleon Kit No-ROX | 5000 x 20 µL rxns | 50 x 1 mL mix, 2 x 1.25 mL buffer |
| Product Code | Description | Pack Size | Presentation |
PB20.14-03 | qPCRBIO SyGreen Chameleon Kit Separate-ROX | 300 x 20 µL rxns | 3 x 1 mL mix, 1 x 200 µL ROX, 1 x 150 µL buffer |
PB20.14-12 | qPCRBIO SyGreen Chameleon Kit Separate-ROX | 1200 x 20 µL rxns | 12 x 1 mL mix, 4 x 200 µL ROX, 4 x 150 µL buffer |
PB20.14-50 | qPCRBIO SyGreen Chameleon Kit Separate-ROX | 5000 x 20 µL rxns | 1 x 50 mL mix, 4 x 520 µL ROX, 2 x 1.25 mL buffer |
PB20.14-51 | qPCRBIO SyGreen Chameleon Kit Separate-ROX | 5000 x 20 µL rxns | 50 x 1 mL mix, 4 x 520 µL ROX, 2 x 1.25 mL buffer |
MATERIAL SAFETY DATA SHEET Chameleon Kit
MSDS Chameleon Kit Lo-ROX
MSDS Chameleon Kit Hi-ROX
MSDS Chameleon Kit No-ROX
MSDS Chameleon Kit Seperate-ROX
Carryover Contamination Prevention
qPCRBIO SyGreen Chameleon Kit UNG
The qPCRBIO SyGreen Chameleon Kit UNG supplies the complete dual-color tracking mix alongside a concentrated 20x UNG/dUTP additive. The integrated uracil N-glycosylase enzymatic step degrades dU-containing PCR products from prior amplifications, preventing carryover contamination without reducing assay sensitivity.
| Product Code | Description | Pack Size | Presentation |
PB21.81-03 | qPCRBIO SyGreen Chameleon Kit Lo-ROX UNG | 300 x 20 µL rxns | 3 x 1 mL mix, 1 x 150 µL buffer, 1 x 200 µL UNG |
PB21.81-12 | qPCRBIO SyGreen Chameleon Kit Lo-ROX UNG | 1200 x 20 µL rxns | 12 x 1 mL mix, 4 x 150 µL buffer, 4 x 200 µL UNG |
PB21.81-50 | qPCRBIO SyGreen Chameleon Kit Lo-ROX UNG | 5000 x 20 µL rxns | 1 x 50 mL mix, 2 x 1.25 mL buffer, 1 x 5 mL UNG |
PB21.81-51 | qPCRBIO SyGreen Chameleon Kit Lo-ROX UNG | 5000 x 20 µL rxns | 50 x 1 mL mix, 2 x 1.25 mL buffer, 1 x 5 mL UNG |
| Product Code | Description | Pack Size | Presentation |
PB21.82-03 | qPCRBIO SyGreen Chameleon Kit Hi-ROX UNG | 300 x 20 µL rxns | 3 x 1 mL mix, 1 x 150 µL buffer, 1 x 200 µL UNG |
PB21.82-12 | qPCRBIO SyGreen Chameleon Kit Hi-ROX UNG | 1200 x 20 µL rxns | 12 x 1 mL mix, 4 x 150 µL buffer, 4 x 200 µL UNG |
PB21.82-50 | qPCRBIO SyGreen Chameleon Kit Hi-ROX UNG | 5000 x 20 µL rxns | 1 x 50 mL mix, 2 x 1.25 mL buffer, 1 x 5 mL UNG |
PB21.82-51 | qPCRBIO SyGreen Chameleon Kit Hi-ROX UNG | 5000 x 20 µL rxns | 50 x 1 mL mix, 2 x 1.25 mL buffer, 1 x 5 mL UNG |
| Product Code | Description | Pack Size | Presentation |
PB21.83-03 | qPCRBIO SyGreen Chameleon Kit No-ROX UNG | 300 x 20 µL rxns | 3 x 1 mL mix, 1 x 150 µL buffer, 1 x 200 µL UNG |
PB21.83-12 | qPCRBIO SyGreen Chameleon Kit No-ROX UNG | 1200 x 20 µL rxns | 12 x 1 mL mix, 4 x 150 µL buffer, 4 x 200 µL UNG |
PB21.83-50 | qPCRBIO SyGreen Chameleon Kit No-ROX UNG | 5000 x 20 µL rxns | 1 x 50 mL mix, 2 x 1.25 mL buffer, 1 x 5 mL UNG |
PB21.83-51 | qPCRBIO SyGreen Chameleon Kit No-ROX UNG | 5000 x 20 µL rxns | 50 x 1 mL mix, 2 x 1.25 mL buffer, 1 x 5 mL UNG |
| Product Code | Description | Pack Size | Presentation |
PB21.84-03 | qPCRBIO SyGreen Chameleon Kit Separate-ROX UNG | 300 x 20 µL rxns | 3 x 1 mL mix, 1 x 200 µL ROX, 1 x 150 µL buffer, 1 x 300 µL UNG |
PB21.84-12 | qPCRBIO SyGreen Chameleon Kit Separate-ROX UNG | 1200 x 20 µL rxns | 12 x 1 mL mix, 4 x 200 µL ROX, 4 x 150 µL buffer, 4 x 300 µL UNG |
PB21.84-50 | qPCRBIO SyGreen Chameleon Kit Separate-ROX UNG | 5000 x 20 µL rxns | 1 x 50 mL mix, 4 x 520 µL ROX, 2 x 1.25 mL buffer, 1 x 5 mL UNG |
PB21.84-51 | qPCRBIO SyGreen Chameleon Kit Separate-ROX UNG | 5000 x 20 µL rxns | 50 x 1 mL mix, 4 x 520 µL ROX, 2 x 1.25 mL buffer, 1 x 5 mL UNG |
MATERIAL SAFETY DATA SHEET Chameleon Kit UNG
MSDS Chameleon Kit UNG Lo-ROX
MSDS Chameleon Kit UNG Hi-ROX
MSDS Chameleon Kit UNG No-ROX
MSDS Chameleon Kit UNG Seperate-ROX
Frequently Asked Questions
Clear answers to common technical, logistics, and evaluation questions regarding the qPCRBIO SyGreen Chameleon Kits.
The system uses two distinct colors: the 2x master mix is supplied blue, while the 100x SampleTrack Buffer is supplied yellow. When the yellow sample mixture is added to the blue mix, the solution shifts immediately to green. This visual change allows operators to verify sample introduction across high-density plate formats at a glance.
No. The tracking dyes are entirely inert and non-inhibitory. They do not alter enzyme activity, thermal cycling requirements, or interfere with SYBR/SYBR-compatible fluorescence detection channels on real-time PCR instruments.
The qPCRBIO SyGreen Chameleon Kits are available in Lo-ROX, Hi-ROX, No-ROX, and Separate-ROX formulations to match the optical calibration requirements of all major real-time PCR platforms. You can consult our instrument compatibility guide or reach out to our application specialists for platform matching.
The standard kit is designed for routine dye-based qPCR applications. The Chameleon Kit UNG includes a concentrated 20x UNG/dUTP system that selectively degrades previously amplified dU-containing PCR products during an initial incubation step, eliminating carryover contamination in high-volume testing workflows.
Yes. Sample kits are available for laboratory validation to verify performance on your target sequences and thermal cycling platforms. Requests can be placed directly through our application specialists using the sample request form.
Kits are offered in standard reaction volumes (300 and 1,200 reactions) as well as bulk packaging options (5,000 reactions supplied in a single 50 mL bottle or multi-vial pouch packs) to support varying throughput requirements and automated liquid handling setups.