qPCRBIO SyGreen® Real time PCR Mixes
qPCRBIO SyGreen Mix uses a proprietary non-inhibitory intercalating dye and antibody-mediated hot start technology for superior sensitivity. Compatible with all standard and fast-cycling qPCR instruments.
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High-Performance qPCR
qPCRBIO SyGreen Mix
This high-sensitivity master mix utilizes a proprietary, non-inhibitory intercalating dye to provide superior signal and sensitivity in real-time PCR. Combined with antibody-mediated hot start technology, it ensures specific amplification and rapid extension rates across all standard and fast cycling platforms. It is engineered for market-leading reproducibility in absolute quantification and gene expression analysis.
| Product Code | Description | Pack Size |
| PB20.11-01 | SyGreen Mix Lo-ROX | 100 x 20 µL rxns (1 x 1 mL) |
| PB20.11-05 | SyGreen Mix Lo-ROX | 500 x 20 µL rxns (5 x 1 mL) |
| PB20.11-06 | SyGreen Mix Lo-ROX | 500 x 20 µL rxns (1 x 5 mL) |
| PB20.11-20 | SyGreen Mix Lo-ROX | 2000 x 20 µL rxns (20 x 1 mL) |
| PB20.11-50 | SyGreen Mix Lo-ROX | 5000 x 20 µL rxns (1 x 50 mL) |
| PB20.11-51 | SyGreen Mix Lo-ROX | 5000 x 20 µL rxns (50 x 1 mL) |
| Product Code | Description | Pack Size |
| PB20.12-01 | SyGreen Mix Hi-ROX | 100 x 20 µL rxns (1 x 1 mL) |
| PB20.12-05 | SyGreen Mix Hi-ROX | 500 x 20 µL rxns (5 x 1 mL) |
| PB20.12-06 | SyGreen Mix Hi-ROX | 500 x 20 µL rxns (1 x 5 mL) |
| PB20.12-20 | SyGreen Mix Hi-ROX | 2000 x 20 µL rxns (20 x 1 mL) |
| PB20.12-50 | SyGreen Mix Hi-ROX | 5000 x 20 µL rxns (1 x 50 mL) |
| PB20.12-51 | SyGreen Mix Hi-ROX | 5000 x 20 µL rxns (50 x 1 mL) |
| Product Code | Description | Pack Size |
| PB20.14-01 | SyGreen Mix Separate-ROX | 100 x 20 µL rxns ([1 x 1 mL mix] & [1 x 200 µL ROX]) |
| PB20.14-05 | SyGreen Mix Separate-ROX | 500 x 20 µL rxns ([5 x 1 mL mix] & [1 x 200 µL ROX]) |
| PB20.14-06 | SyGreen Mix Separate-ROX | 500 x 20 µL rxns ([1 x 5 mL mix] & [1 x 200 µL ROX]) |
| PB20.14-20 | SyGreen Mix Separate-ROX | 2000 x 20 µL rxns ([20 x 1 mL mix] & [4 x 200 µL ROX]) |
| PB20.14-50 | SyGreen Mix Separate-ROX | 5000 x 20 µL rxns ([1 x 50 mL mix] & [2 x 520 µL ROX]) |
| PB20.14-51 | SyGreen Mix Separate-ROX | 5000 x 20 µL rxns ([50 x 1 mL mix] & [2 x 520 µL ROX]) |
| Product Code | Description | Pack Size |
| PB20.13-01 | SyGreen Mix With Fluorescein | 100 x 20 µL rxns (1 x 1 mL) |
| PB20.13-05 | SyGreen Mix With Fluorescein | 500 x 20 µL rxns (5 x 1 mL) |
| PB20.13-06 | SyGreen Mix With Fluorescein | 500 x 20 µL rxns (1 x 5 mL) |
| PB20.13-20 | SyGreen Mix With Fluorescein | 2000 x 20 µL rxns (20 x 1 mL) |
Enhanced Visualization
qPCRBIO SyGreen Blue Mix
Featuring a non-reactive blue dye, this variant improves pipetting accuracy by providing clear visualization of samples during reaction setup. The formulation maintains the same advanced enzyme and buffer chemistry as the standard SyGreen Mix, offering exceptional detection of low copy number targets and complex templates. It is compatible with all real-time PCR instruments and supports both standard and fast cycling conditions.
| Product Code | Description | Pack Size |
| PB20.15-01 | SyGreen Blue Mix Lo-ROX | 100 x 20 µL rxns (1 x 1 mL) |
| PB20.15-05 | SyGreen Blue Mix Lo-ROX | 500 x 20 µL rxns (5 x 1 mL) |
| PB20.15-20 | SyGreen Blue Mix Lo-ROX | 2000 x 20 µL rxns (20 x 1 mL) |
| PB20.15-50 | SyGreen Blue Mix Lo-ROX | 5000 x 20 µL rxns (1 x 50 mL) |
| PB20.15-51 | SyGreen Blue Mix Lo-ROX | 5000 x 20 µL rxns (50 x 1 mL) |
| Product Code | Description | Pack Size |
| PB20.16-01 | SyGreen Blue Mix Hi-ROX | 100 x 20 µL rxns (1 x 1 mL) |
| PB20.16-05 | SyGreen Blue Mix Hi-ROX | 500 x 20 µL rxns (5 x 1 mL) |
| PB20.16-20 | SyGreen Blue Mix Hi-ROX | 2000 x 20 µL rxns (20 x 1 mL) |
| PB20.16-50 | SyGreen Blue Mix Hi-ROX | 5000 x 20 µL rxns (1 x 50 mL) |
| PB20.16-51 | SyGreen Blue Mix Hi-ROX | 5000 x 20 µL rxns (50 x 1 mL) |
| Product Code | Description | Pack Size |
| PB20.17-01 | SyGreen Blue Mix Separate-ROX | 100 x 20 µL rxns (+ ROX) |
| PB20.17-05 | SyGreen Blue Mix Separate-ROX | 500 x 20 µL rxns (+ ROX) |
| PB20.17-20 | SyGreen Blue Mix Separate-ROX | 2000 x 20 µL rxns (+ ROX) |
| PB20.17-50 | SyGreen Blue Mix Separate-ROX | 5000 x 20 µL rxns (+ ROX) |
| PB20.17-51 | SyGreen Blue Mix Separate-ROX | 5000 x 20 µL rxns (50 x 1 mL) |
Technical Specifications
Essential performance data, chemistry specs, and hardware compatibility for the experienced user.
The error rate is approximately 1 in 200,000 nucleotides (1 x 10^-5).
No, it is a next-gen asymmetric cyanine dye that is non-inhibitory at high concentrations, providing more consistent melt curves.
Lo-ROX (112 nM), Hi-ROX (1.12 µM), and Separate-ROX (50 µM additive).
Yes, it is fully compatible with bisulfite-treated templates for methylation analysis.
The 3 mM final Magnesium Chloride (MgCl2) concentration in the mix is high enough to compensate for any minor chelation caused by 1x TE storage buffers.
- Non-inhibitory SyGreen chemistry for ultra-stable melt curves.
- 2x Mastermix—just add primers, template, and water.
- Minimal non-specific amplification with advanced HS-Taq.
- Fully compatible with TA cloning and sequencing.
- High tolerance for template-derived inhibitors and TE buffer.
- Non-inhibitory SyGreen chemistry for ultra-stable melt curves.
- 2x Mastermix—just add primers, template, and water.
- Minimal non-specific amplification with advanced HS-Taq.
- Fully compatible with TA cloning and sequencing.
- High tolerance for template-derived inhibitors and TE buffer.
Optimization & Troubleshooting
Guidance on protocol adjustments, efficiency recovery, and signal normalization.
While 2 minutes at 95°C is recommended for maximum stringency, the Hot-Start Taq can be activated in as little as 10 seconds for standard, low-complexity templates.
To stop DNA from sticking to polypropylene tubes, dilute your standards in a buffer of 10 mM Tris-HCl (pH 8.0) containing 0.05% Tween-20. Avoid freezing standards after dilution.
This is usually due to high enzymatic efficiency. To confirm it isn't non-specific amplification, check for a single peak in your melt analysis. If multiple peaks appear, increase your annealing temperature.
High background is often caused by an excess of template DNA. Diluting your samples 100x to 1000x typically resolves the issue by reducing the amount of non-target DNA the dye binds to.
Yes. The enzyme produces 3'-d(A)-overhangs. We recommend using a standard spin-column clean-up kit before ligation to remove the intercalating dye and buffer salts.