seqWell AgriPrep™ Library Prep Kit
The AgriPrep™ Library Prep Kit is engineered specifically for the demands of applied agrigenomics, based on the one-step ExpressPlex technology. Utilizing a one-step tagmentation and amplification workflow, it enables researchers to move from extracted genomic DNA to sequence-ready libraries in just 100 minutes. The system is optimized for low-pass whole genome sequencing (LP-WGS) and SKIM-sequencing, providing a cost-effective alternative to traditional microarrays and high-depth WGS.
| Feature | Specification |
| Primary Applications | Genotyping-by-sequencing (GBS), Low-pass WGS, SKIM-seq |
| Enzyme Technology | TnX™ Next-generation engineered transposase |
| Total Workflow Time | 100 minutes |
| Hands-on Time | 30 minutes |
| DNA Input Range | Working: 10 - 200 ng; Normalization: 20 - 100 ng |
| Indexing | CDI (Up to 3,072 combinations) |
| Format | 96-well Custom dispense; Bulk reagent format available |
| Article Number | Description | Size |
| 301737 | AgriPrep™ Library Prep Kit (any index set) | 1x96rxn |
| 301729 | AgriPrep™ Library Prep Kit (any index set) | 4x96rxn |
| 301721 | AgriPrep™ Library Prep Kit - Index Set 1000 | 4x96rxn |
| 301722 | AgriPrep™ Library Prep Kit - Index Set 2000 | 4x96rxn |
| 301723 | AgriPrep™ Library Prep Kit - Index Set 3000 | 4x96rxn |
| 301724 | AgriPrep™ Library Prep Kit - Index Set 4000 | 4x96rxn |
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Efficiency by Design
Auto-Normalization & Early Pooling
AgriPrep™ features integrated auto-normalization of mean insert size and read depth. This eliminates the need for individual sample quantification and dilution before pooling, significantly reducing the labor-intensive QC steps typically required for large-scale genotyping. The workflow is highly tolerant of variable DNA input, maintaining uniform coverage across hundreds or thousands of samples in a single run.
- Genotyping-by-sequencing (GBS)
- Low pass sequencing
- SKIM-seq
- Genotyping-by-sequencing (GBS)
- Low pass sequencing
- SKIM-seq
Cut Sequencing Waste by 80% with AgriPrep’s Auto-Normalizing Chemistry.
Smarter Normalization, Lower Costs
Stop overspending on redundant sequencing to compensate for inconsistent data. The AgriPrep™ system utilizes integrated auto-normalization of read depth, which removes the grueling labor of manual quantification and sample dilution. By significantly narrowing the variance in coverage across samples, you can achieve your required depth without the excessive costs of over-sequencing.
Achieving an 80% Reduction in Sequencing Requirements
The efficiency of auto-normalization is clearly demonstrated when comparing AgriPrep against standard library preparation methods. In a performance test using DNA inputs ranging from 20 to 100 ng:
- AgriPrep™ Advantage: By pooling samples by equal volume, the AgriPrep workflow produced highly uniform read counts. For a pool sequenced to 1.2 million reads, every sample successfully reached the target threshold of ≥100,000 reads.
- The Cost of Non-Normalizing Methods: In contrast, standard prep methods exhibited such high variance that the same pool would require 6 million total reads (a 5-fold increase) just to ensure the lowest-performing sample met the same 100,000-read minimum.
By choosing AgriPrep™, you ensure that every sample is represented equally, allowing you to maximize your flow cell capacity and drastically lower your cost-per-sample.
Special Features
SIMPLE WORKFLOW
- One-step tagmentation & amplification
- Auto-normalization
- Early sample pooling
- 100-minute workflow, 30 minutes hands-on
SCALABLE
- Automation-friendly
- Up to 3072 indexes
- 384-well plate format compatibility
- Available in bulk reagent format
AFFORDABLE
- Up to 95% reduction in consumables
- Significant labor savings
- Easily miniaturized
Agrigenomic Solutions
Actionable Insights for Breeding
Genomic Selection & Trait Mapping
Rapidly identify markers and traits across large populations to inform breeding decisions.
Parentage & Variant Discovery
High-multiplexing capabilities support large-scale screening for parentage analysis and genetic diversity studies.
Low-Pass WGS (LP-WGS)
Achieve genome-wide coverage at a fraction of the cost of traditional whole-genome sequencing methods.
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Ready to Scale Your Agrigenomics Workflow?
Contact our specialists to discuss custom index combinations (up to 3,072) and automation-friendly bulk reagent options.
