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seqWell AgriPrep™ Library Prep Kit

Simplified, scalable library preparation for high-throughput plant and animal genomics.
seqWell NGS Library Prep & Controls

The AgriPrep™ Library Prep Kit is engineered specifically for the demands of applied agrigenomics, based on the one-step ExpressPlex technology. Utilizing a one-step tagmentation and amplification workflow, it enables researchers to move from extracted genomic DNA to sequence-ready libraries in just 100 minutes. The system is optimized for low-pass whole genome sequencing (LP-WGS) and SKIM-sequencing, providing a cost-effective alternative to traditional microarrays and high-depth WGS.

FeatureSpecification
Primary ApplicationsGenotyping-by-sequencing (GBS), Low-pass WGS, SKIM-seq
Enzyme TechnologyTnX™ Next-generation engineered transposase
Total Workflow Time100 minutes
Hands-on Time30 minutes
DNA Input RangeWorking: 10 - 200 ng; Normalization: 20 - 100 ng
IndexingCDI (Up to 3,072 combinations)
Format96-well Custom dispense; Bulk reagent format available
Article NumberDescriptionSize
301737

AgriPrep™ Library Prep Kit (any index set)

1x96rxn
301729AgriPrep™ Library Prep Kit (any index set)4x96rxn
301721AgriPrep™ Library Prep Kit - Index Set 10004x96rxn
301722AgriPrep™ Library Prep Kit - Index Set 20004x96rxn
301723AgriPrep™ Library Prep Kit - Index Set 30004x96rxn
301724AgriPrep™ Library Prep Kit - Index Set 40004x96rxn

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Efficiency by Design

Auto-Normalization & Early Pooling

AgriPrep™ features integrated auto-normalization of mean insert size and read depth. This eliminates the need for individual sample quantification and dilution before pooling, significantly reducing the labor-intensive QC steps typically required for large-scale genotyping. The workflow is highly tolerant of variable DNA input, maintaining uniform coverage across hundreds or thousands of samples in a single run.

  • Genotyping-by-sequencing (GBS)
  • Low pass sequencing
  • SKIM-seq
  • Genotyping-by-sequencing (GBS)
  • Low pass sequencing
  • SKIM-seq

Cut Sequencing Waste by 80% with AgriPrep’s Auto-Normalizing Chemistry.

Smarter Normalization, Lower Costs

Stop overspending on redundant sequencing to compensate for inconsistent data. The AgriPrep™ system utilizes integrated auto-normalization of read depth, which removes the grueling labor of manual quantification and sample dilution. By significantly narrowing the variance in coverage across samples, you can achieve your required depth without the excessive costs of over-sequencing.

Achieving an 80% Reduction in Sequencing Requirements

The efficiency of auto-normalization is clearly demonstrated when comparing AgriPrep against standard library preparation methods. In a performance test using DNA inputs ranging from 20 to 100 ng:

  • AgriPrep™ Advantage: By pooling samples by equal volume, the AgriPrep workflow produced highly uniform read counts. For a pool sequenced to 1.2 million reads, every sample successfully reached the target threshold of ≥100,000 reads.
  • The Cost of Non-Normalizing Methods: In contrast, standard prep methods exhibited such high variance that the same pool would require 6 million total reads (a 5-fold increase) just to ensure the lowest-performing sample met the same 100,000-read minimum.

By choosing AgriPrep™, you ensure that every sample is represented equally, allowing you to maximize your flow cell capacity and drastically lower your cost-per-sample.

Special Features

SIMPLE WORKFLOW

  • One-step tagmentation & amplification
  • Auto-normalization
  • Early sample pooling
  • 100-minute workflow, 30 minutes hands-on

SCALABLE

  • Automation-friendly
  • Up to 3072 indexes
  • 384-well plate format compatibility
  • Available in bulk reagent format

AFFORDABLE

  • Up to 95% reduction in consumables
  • Significant labor savings
  • Easily miniaturized

Agrigenomic Solutions

Actionable Insights for Breeding

Genomic Selection & Trait Mapping

Rapidly identify markers and traits across large populations to inform breeding decisions.

Parentage & Variant Discovery

High-multiplexing capabilities support large-scale screening for parentage analysis and genetic diversity studies.

Low-Pass WGS (LP-WGS)

Achieve genome-wide coverage at a fraction of the cost of traditional whole-genome sequencing methods.

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Ready to Scale Your Agrigenomics Workflow?

Contact our specialists to discuss custom index combinations (up to 3,072) and automation-friendly bulk reagent options.

Technical Resources

seqWell AgriPrep™ User Guide

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seqWell AgriPrep™ Master Index List

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seqWell XLEAP guidelines

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Downloads

AgriPrep™ Library Prep Kit Brochure

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