seqWell ExpressPlex 2.0 Library Prep Kit
ExpressPlex 2.0 is an automation-friendly library preparation solution designed for high-throughput sequencing of synthetic constructs, plasmids, and amplicons. Utilizing the engineered TnX™ transposase, it combines fragmentation, barcoding, and amplification into a single 100-minute workflow. The system features built-in auto-normalization, which ensures consistent read depth across a 40-fold range of DNA input, eliminating the need for individual sample quantification prior to pooling.
| Feature | Specification |
| Primary Applications | Plasmid, amplicon |
| Transposase | TnX™ (Engineered for reduced bias) |
| Workflow Time | 100 minutes total ( <30 minutes hands-on) |
| Input Requirement | 96-well format: 384-well format: |
| Fragment Range | 400 – 1,200 bp |
| Indexing | Combinatorial Dual Indexing (Up to 6,144 combinations) |
| Compatibility | All Illumina platforms; Element AVITI™ & Complete Genomics (via conversion) |
| Article Number | Description | Size |
| 301170 | ExpressPlex 2.0- 96-well (any Index Set) | 1x96rxn |
| 301180 | ExpressPlex 2.0- 96-well (any Index Set) | 4x96rxn |
| 301176 | ExpressPlex 2.0- 96-well (Index Set 1000) | 4x96rxn |
| 301177 | ExpressPlex 2.0- 96-well (Index Set 2000) | 4x96rxn |
| 301178 | ExpressPlex 2.0- 96-well (Index Set 3000) | 4x96rxn |
| 301179 | ExpressPlex 2.0- 96-well (Index Set 4000) | 4x96rxn |
| 301152 | ExpressPlex 2.0- 384-well (any Index Set) | 1x384rxn |
| 301159 | ExpressPlex 2.0- 384-well (any Index Set) | 4x384rxn |
| 301542 | Custom ExpressPlex 2.0 - 96-well (any Index Set) | 1x96rxn |
| 301546 | Custom ExpressPlex 2.0 - 96-well (any Index Set) | 4x96rxn |
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- Purified plasmid DNA
- RCA-amplified DNA
- Colony PCR amplicons
- Amplicons >350 bp
- Microbial genomic DNA
- Purified plasmid DNA
- RCA-amplified DNA
- Colony PCR amplicons
- Amplicons >350 bp
- Microbial genomic DNA
Advanced Enzyme Engineering
Enhanced Performance with TnX™ Transposase
The core of ExpressPlex 2.0 is the TnX™ transposase, specifically engineered to provide superior coverage uniformity compared to standard Tn5-based methods. This next-generation enzyme reduces insertion bias and improves GC profile coverage, resulting in higher library complexity and fewer gaps in critical regions of interest.
The average fragment size ranges from 400-1200bp. When directly compared to a Tn5-based competitor library prep kit for sequencing of 2 different plasmids, ExpressPlex 2.0 produced significantly lower CVs and range of coverage (max/min).
Optimized for Synthetic Biology and Genomics
Plasmid & Synthetic Constructs
Rapidly screen synthetic biology discovery libraries with high-level multiplexing.
Amplicon Sequencing
Streamlined preparation of amplicons >350bp for genetic variant analysis and mutation detection.
Expert Support
Optimize Your NGS Library Prep
Have questions about integrating ExpressPlex 2.0 into your high-throughput laboratory workflow? Our product specialists are ready to provide technical guidance and customized quotes.
Applications, Compatibility & Protocol Optimization
Technical guidance on sample input ranges, instrumentation compatibility, and library multiplexing for the ExpressPlex™ 2.0 system.
The ExpressPlex™ 2.0 library preparation kit is recommended for amplicons, plasmids, and other small targets.
While ExpressPlex 2.0 has not been validated for use in RNA-seq, users have been able to obtain sufficient RNA-seq data for their purposes. This may require further optimization depending on your project requirements and seqWell is only able to fully support the kit for DNA-seq applications. We have not validated a specific cDNA kit, but you will need to first generate cDNA amplicons of >350 bps size and >1ng/μl yield as inputs. If the cDNA amplicons generated are <500 bps in size, you may want to use a higher amount of input ~5-10ng/μl to avoid over-tagmentation. As EP2.0 is not designed for RNA-seq applications it will not retain stranded information from your RNA.
Yes. The ExpressPlex™ 2.0 kit includes all the indexed adapters, amplification master mix, and amplification primers necessary to make dual-indexed Illumina-compatible libraries.
Start off the library preparation with the plates provided. After the reactions are mixed by pipette, seal, and centrifuge the plate. Then, transfer the reactions to a plate compatible with your thermal cycler. Seal the new reaction plate and centrifuge again before loading into your thermal cycler. Processing it this way is critical to ensure proper proportions of reagents are used and normalization is successful.
- ExpressPlex™ 2.0 reagents can be saved if they are as-shipped (i.e. if no DNA or Indexing reagents have been added, and no incubations have been done). If processing <96 samples for your 96-well kit plate, and you would like to preserve the reagents, set the reaction up as described in the User Guide.
- With a razor blade, cut the seals up to the sample number being processed. Only peel the heat seal from the wells of the Indexing Reagent Plate and Ready Reaction Plate corresponding to the total number of samples that will be processed. Proceed with both sample transfer and Indexing Reagent transfer into the Ready Reaction plate wells in use, pipette mix, seal, and centrifuge. Unseal the plate and transfer the reactions to a new PCR plate. Cover up the used wells to prevent contamination and store remaining reagents at -20ºC for subsequent use.
- For the ExpressPlex™ 2.0 (96-well) Library Prep Kit, up to 1536 index combinations are available. If more than 1536 index combinations are required, please contact us.
- For the ExpressPlex™ 2.0 (384-well) Library Prep Kit, up to 6144 index combinations are available.
No, the seals are not pierceable. You will need to peel back the seal to access the wells. We have done extensive testing to confirm no contamination issues if plates are properly centrifuged before peeling.
seqWell recommends globally diluting samples to bring the average DNA concentration of the samples within our concentration range (0.25 - 10 ng/µl). Use of lower or higher DNA concentration may adversely affect sequencing performance.
The ExpressPlex™ 2.0 (96-well) library preparation kit performs optimally with 5 ng of dsDNA per reaction (2.5 ng of dsDNA per reaction for 384-well format). However, individually normalizing each sample to 1.25 ng/µl is not necessary as ExpressPlex 2.0 library preparation kits are formulated to tolerate up to a 40-fold difference in sample input (1 to 40 ng for 96-well format; 0.5 to 20 ng for 384-well format).