seqWell MosaiX™ Library Prep Kit
The MosaiX™ DNA Library Prep Kit utilizes proprietary directional tagmentation technology to combine the speed of transposase-based workflows with the library quality typically associated with ligation methods. Powered by the engineered TnX™ transposase, it minimizes insertion-site bias and maximizes the production of functional library molecules.
| Feature | Specification |
| Technology | Directional Tagmentation (TnX™ Transposase) |
| Total Protocol Time | 90 minutes |
| Hands-on Time | < 35 minutes |
| DNA Input Range | 1 – 50 ng (5 – 50 ng optimal) |
| Sequencer Compatibility | All Illumina platforms; Element AVITI™ & Complete Genomics (with conversion) |
| Mean Insert Size WGS | 350 - 500 bp |
| Mean Insert Size Hybrid Capture | 250 - 350 bp |
| Article Number | Description | Size |
| 301458 | MosaiX™ v2 Kit | 24rxn |
| 301464 | MosaiX™ v2 Kit | 96rxn |
| 301429 | MosaiX™ Compatible UDI Index Primers | 96rxn |
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Engineered Enzyme Technology
The TnX™ Transposase Advantage
Traditional Tn5-based methods often suffer from high insertion bias and non-productive fragments. MosaiX uses TnX™, an engineered transposase evolved for superior activity and reduced sequence-dependent bias. This results in more uniform genome coverage and higher molecular complexity, even when working with challenging samples like FFPE-derived DNA.
- Human whole genome sequencing (WGS)
- Human whole exome sequencing (WES)
- Human germline target capture panels
- Plant & animal WGS and target capture panels
- Human whole genome sequencing (WGS)
- Human whole exome sequencing (WES)
- Human germline target capture panels
- Plant & animal WGS and target capture panels
High-Complexity WGS Libraries in a Fraction of the Time
The MosaiX™ Revolution
The seqWell MosaiX™ 90-minute workflow represents a significant leap in NGS library preparation by bridging the gap between the speed of tagmentation and the high-quality data typically reserved for ligation-based methods. At its core is the engineered TnX transposase, which has been evolved to drastically reduce the sequence-dependent insertion bias common in traditional Tn5 methods.
The process is remarkably efficient, requiring only 35 minutes of hands-on time. It utilizes a proprietary directional tagmentation chemistry that ensures every tagging event produces a functional, sequencer-ready molecule (with the proper P5 and P7 orientations), effectively doubling the molecular complexity of the library compared to older tagmentation kits. This streamlined approach allows researchers to go from genomic DNA to a PCR-ready library in just 45 minutes, with the entire protocol—including purification and amplification—finishing in an hour and a half.
Technical Consultation
Optimize Your Library Preparation
Speak with a specialist to learn how the MosaiX™ kit can improve your specific NGS workflow and sample types.
Applications & Reagent Compatibility
Frequently asked questions regarding the intended use, buffer stability, and kit components for the MosaiX™ Library Prep series.
- Human whole genome sequencing (WGS)
- Human whole exome sequencing (WES)
- Human germline target capture panels
- Plant & animal WGS and target capture panels
The kit is highly robust and compatible with DNA diluted in 10 mM Tris-HCl, 1X TE, Low TE, or Water. Its fragmentation profiles remain unaffected by EDTA.
Kits include TnX Read 1 Tagging Reagent, Tagmentation Enhancer, Read 2 Adapter, Ligase, Reaction Buffers, 2X Amplification Master Mix, and MAGwise purification beads.