WatchMaker Genomics DNA Library Prep Kits
Watchmaker DNA Library Prep Kits are engineered to maximize the conversion of template DNA into sequenceable molecules. By improving library complexity and reducing bias, these kits enable higher sensitivity in challenging applications like liquid biopsy and FFPE analysis.
| Feature | Specification |
| Input Range | 500 pg to 1 µg (Standard); <1 ng to 500 ng (Fragmentation) |
| Workflow Time | < 2 hours (Standard); < 90 minutes (Fragmentation, PCR-free) |
| Artifact Reduction | Up to 90% reduction in chimeric reads and hairpins |
| Polymerase | Equinox high-fidelity polymerase |
| Product Name | 24 RXN | 96 RXN | 384 RXN |
| DNA Library Prep Kit (with fragmentation) | 7K0019-024 | 7K0019-096 | 7K0019-384 |
| DNA Library Prep Kit (with fragmentation, PCR-free) | 7K0013-024 | 7K0013-096 | 7K0013-384 |
| DNA Library Prep Kit (standard) | 7K0102-024 | 7K0102-096 | 7K0102-384 |
| DNA Library Prep Kit (standard, PCR-free) | 7K0101-024 | 7K0101-096 | 7K0101-384 |
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Choose Your Library Prep Solution
DNA Library Prep Kit (Standard)
Designed for samples that are already fragmented, such as cfDNA, or for workflows where mechanical shearing (sonication) is preferred.
DNA Library Prep with Fragmentation
Ideal for intact genomic DNA. Features highly tunable enzymatic fragmentation that preserves low-input samples and simplifies automation by eliminating sonication.
User Guides
User Guide: Watchmaker DNA Library Prep Kit with Fragmentation
User Guide: Watchmaker DNA Library Prep Kit
Watchmaker Sequenomics: Redefining NGS Efficiency
Discover the "Sequenomics" approach—where advanced biochemistry meets intentional workflow design. This video demonstrates how integrating high-performance enzymes with automated, streamlined protocols reduces manual intervention and maximizes sequencing output for high-throughput laboratories.
- Up to 90% reduction in sequence artifacts
- 20-30% higher mean coverage for cfDNA
- Streamlined workflows: PCR-free libraries in < 90 minutes
- Highly tunable fragmentation from < 1 ng to 500 ng
- Improved sensitivity for liquid biopsy and FFPE samples
- Powered by Equinox ultra-high-fidelity polymerase
- Up to 90% reduction in sequence artifacts
- 20-30% higher mean coverage for cfDNA
- Streamlined workflows: PCR-free libraries in < 90 minutes
- Highly tunable fragmentation from < 1 ng to 500 ng
- Improved sensitivity for liquid biopsy and FFPE samples
- Powered by Equinox ultra-high-fidelity polymerase
Technical Insights & Kit Selection
Guidance on selecting the optimal library prep chemistry for your sample type, input requirements, and workflow goals.
Use the Standard kit for pre-fragmented DNA, such as cfDNA, CT-DNA, or samples already sheared via sonication. Use the Fragmentation kit for intact genomic DNA or FFPE samples where enzymatic shearing is preferred.
The Standard kit supports inputs from 500 pg to 1 µg. The Fragmentation kit is optimized for 1 ng to 500 ng. Both are compatible with low-input and high-complexity library requirements.
The proprietary fragmentation and ERAT module reduces sequence artifacts—specifically false chimeras and hairpins—by up to 90%. This results in more accurate variant calling and better sequencing economy.
Yes. Both kits use a single-tube, streamlined protocol with minimal transfer steps. Reagent overages are included to account for dead volume, making them ideal for high-throughput automated platforms.
Both kits support PCR-free library preparation provided the starting material is of sufficient mass and quality. For samples requiring expansion, the included Equinox high-fidelity polymerase ensures low-bias amplification.