Watchmaker Genomics RNA library Preparation kits
There is a need for RNA library preparation solutions that are highly sensitive, scalable, robust with respect to performance with challenging clinically relevant sample types, such as FFPE and low inputs.
Transcriptomics provides a snapshot of cellular activity at specific points in time and in response to environmental stimuli. It aims to characterize both protein-coding and non-coding transcripts (and the variation therein) and quantify their complex ebb and flow allowing researchers to unravel biological mechanisms, detect and monitor eg. cancer, and validate biomarkers and drug responses.
WatchMaker Genomics' portfolio delivers in all of these areas, enabling cutting edge research and applications in the transcriptomics space.
Watchmaker RNA Library Prep Kits with Polaris® Depletion for improved sensitivity with FFPE and low-inputs using a rapid library prep workflow
Watchmaker mRNA Library Prep Kits for library prep in < 5 hours with as little as 2.5 ng
Watchmaker RNA Library Prep Kits for the streamlined prep of total RNA libraries for target enrichment applications
| Feature | Specification |
| Input Range | 0.25 ng – 1 µg Total RNA |
| Turnaround Time | < 3.5 to 5 hours (workflow dependent) |
| Enzyme Technology | StellarScript™ Reverse Transcriptase |
| Sample Compatibility | Intact RNA, FFPE, Whole Blood |
| Article Number | Description |
| 7K0001-024 | mRNA Library Prep Kit (24 rxn |
| 7K0001-096 | mRNA Library Prep Kit (96 rxn) |
| 7K0078-024 | RNA Library Prep Kit (24 rxn) |
| 7K0078-096 | RNA Library Prep Kit (96 rxn) |
| 7BK0002-024 | RNA Prep Kit with Polaris Depletion (24 rxn) |
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Key Technical Features
Reduced Bias & High Complexity
Maintains quantitative gene expression profiles and detects more unique genes than standard commercial kits, particularly at low inputs.
FFPE Optimized
Includes a novel FFPE treatment step to address damage and cross-linking, salvaging usable data from clinically relevant samples.
Automation-Ready
Designed with minimal cleanup/handling steps and generous reagent overages for seamless integration on liquid handling platforms.
RNA Portfolio Overview
| RNA Prep Kit with Polaris Depletion | mRNA Prep Kit | RNA Prep Kit | |
| Gene expression analysis | X | X | |
| Gene fusion detection | X | X | X |
| Isoform/ernative splicing analysis | X | X | |
| Novel transcript discovery | X | X | |
| Single nucleotide variant (SNV) detection | X | X | X |
| Long non-coding RNA-seq | X | ||
| Targeted sequencing | X |
Watchmaker Sequenomics: Redefining NGS Efficiency
Discover the "Sequenomics" approach—where advanced biochemistry meets intentional workflow design. This video demonstrates how integrating high-performance enzymes with automated, streamlined protocols reduces manual intervention and maximizes sequencing output for high-throughput laboratories.
Technical Insights & Kit Selection
Guidance on selecting the optimal library prep chemistry for your sample type, input requirements, and workflow goals.
Selection depends on your research goals and sample quality. Choose mRNA Library Prep for focused coding-region analysis of high-quality (RIN >7) eukaryotic samples. Select RNA Library Prep (Total) for targeted sequencing or hybridization capture from ultra-low inputs (0.25 ng). Use RNA Prep with Polaris Depletion for whole-transcriptome sequencing from blood or degraded samples where removal of rRNA and globin transcripts is required.
Watchmaker kits include a specialized FFPE Treatment Buffer designed to address chemical damage and cross-linking inherent in fixation. When paired with the engineered StellarScript™ Reverse Transcriptase, this module enhances conversion efficiency, allowing for the generation of high-complexity libraries from samples that typically fail with standard RT enzymes.
The Polaris Depletion module is specifically optimized for Human, Mouse, and Rat (HMR) samples. It efficiently removes cytoplasmic and mitochondrial rRNA across all three species, while its globin-specific probes are specifically designed for Human transcripts.
Yes. The workflows are designed with fewer bead cleanups and "on-magnet" washing steps to minimize handling. Watchmaker provides 96-reaction kits with generous overages to accommodate dead volumes on liquid handling platforms like the Biomek i7.
These kits utilize random priming for first-strand synthesis to ensure even coverage across the transcript, which typically produces library inserts of ~200 bp. Because they do not generate full-length cDNA, they are not recommended for native long-read sequencing applications.