Yourgene Health LightBench Series
The LightBench Series replaces multiple standalone benchtop instruments by unifying DNA size selection, automated fragment length analysis, and solution-based fluorometric quantification into a single automated platform. Designed to eliminate workflow bottlenecks in Next-Generation Sequencing (NGS), long-read sequencing, synthetic biology, and quality control pipelines, these systems ensure target enrichment with reproducibility and sensitivity.
| Parameter | LightBench | LightBench Discover | NIMBUS Select (Automation Extension) |
| Target DNA Size Range | 0 to 20 kb+ (Size Selection) | 0 to 20 kb+ (Size Selection); 50 bp to 150 kb (Fragment Analytics) | 100 bp to 20,000 bp |
| Size Selection Resolution | 10% – 20% | 10% – 20% | High resolution (<3.5% CV) |
| Intrinsic Recovery Efficiency | 70% – 80% | 70% – 80% | 70% – 90% (Average ~70%) |
| Throughput per Run | Up to 12 channels (Size Selection); Up to 48 samples (Analytics) | Up to 12 channels (Size Selection); Up to 48 samples (Analytics) | Up to 96 samples |
| Size Selection Run Time | 1 to 4 hours (application dependent) | 1 to 4 hours (application dependent) | 30 to 200 minutes |
| Pulsed-Field Capability | No | Yes (large fragment analytics up to 150 kb) | No |
| Quantification Detection Limit | 0.2 ng (PicoGreen / fluorescence assay) | 0.2 ng (PicoGreen / fluorescence assay) | Integrated Fluorometric Assays |
| Quantification Accuracy | ±15% | ±15% | ±15% |
| Quantification Throughput | 1 to 384 samples (<30 mins for 384-well) | 1 to 384 samples (<30 mins for 384-well) | Plate-based integration |
| Physical Dimensions (W x D x H) | 32.5 cm x 42.4 cm x 38.1 cm | 32.5 cm x 42.4 cm x 38.1 cm | Robotic Workstation Footprint |
| Weight | 20 kg | 20.5 kg | Workstation dependent (~500 lbs rated bench) |
| Product Code | Description |
| CG-12500-05 | Yourgene LightBench Discover Instrument (automated gel electrophoresis system) |
| CG-11806-06 | LightBench Discover Base Unit |
| CG-12491-30 | LightBench Discover Install Kit (camera calibration block & PQ reagents) |
| CG-14100-13-050-31-31/16 | Ranger ICF Size Selection Kit (16 cassettes, 12 samples/cassette, 0.5% agarose) |
| CG-14500-20-050-24-DL/16 | Analytics Kit (16 cassettes, up to 75 kb quantitative / 150 kb qualitative sizing) |
| CG-14550-24-DL | Dual Dye Loading Buffer with 2 kb marker & Large Fragment Analytics DNA Ladder |
| CG-14510-01 | LightBench Discover Cassette Reuse Kit |
| CG-14192-01 | 384-well Plates (Pack of 50) |
Resources
Have product questions?
Base Platform
LightBench: Standardized 3-in-1 Benchtop Performance
The standard LightBench system delivers automated agarose gel electrophoresis designed to streamline NGS sample preparation and synthetic biology workflows. By combining DNA size selection (0 to 20 kb+), fragment length analysis, and plate-based fluorometric mass quantification into a single compact unit, it replaces up to three standalone laboratory instruments. The platform features dynamic real-time imaging, integrated internal reference markers, and electronic voltage control to achieve greater than 97% size selection reproducibility and average target yields exceeding 70%. It connects directly with robotic platforms and operates using pre-cast agarose cassettes, significantly reducing setup times and operational costs in high-throughput QC, cloning, and NIPT environments.
Extended Capabilities
LightBench Discover: High-Molecular-Weight & Large Fragment Analytics
Powered by Ranger Technology, the LightBench Discover expands benchtop gel electrophoresis into long-read sequencing applications and advanced genomic quality control. Incorporating field inversion technology alongside pre-cast agarose slab cassettes, the system performs precise fragment length analysis for high-molecular-weight DNA up to 150 kb. It delivers complete 3-in-1 functionality—targeted size selection, pulsed-field large fragment analytics, and plate-based fluorometric quantification—within less than 2 hours. Dedicated Ranger analytics software provides real-time visualization, automated electropherogram generation, and mass/molarity-based quality metrics to ensure optimal sample integrity prior to downstream sequencing.
Automated Workstation
NIMBUS Select: Walkaway Scale for High-Throughput Laboratories
The NIMBUS Select workstation combines Ranger Technology electrophoresis hardware with a 96-channel liquid handling platform to deliver complete walkaway automation for high-volume laboratories. Capable of processing up to 96 samples per run in parallel across two on-deck electrophoresis pedestals, it automates sample loading, real-time separation tracking, target extraction, and post-run liquid transfers. Operating with high precision (>95% accuracy, <3.5% CV size distribution), the workstation dramatically reduces manual labor in large-scale gene synthesis, production NGS library preparation, and high-throughput sequencing operations.
Instructions for Use
Yourgene Health IFU: Quick Start Guide DNA Size Selection with Flow Through Cassettes
Yourgene Health IFU: Quick Start Guide LightBench Discover Large DNA Fragment Length Analytics
Yourgene Health IFU: Quick Start Guide DNA Fragment Length Analytics Under 20 kb
Yourgene Health IFU: Quick Start Guide DNA Size Selection with In Channel Filter Cassettes
Yourgene Health IFU: Quick Start Guide DNA Size Selection for PacBio Sequencing
Yourgene Health IFU: Quick Start Guide Plate Based Quantification
Frequently Asked Questions
Common technical and operational inquiries regarding the LightBench Series and Ranger Technology in NIPT and NGS library preparation workflows.
The LightBench Series uses automated gel electrophoresis powered by Ranger Technology to perform tight DNA size selection. By precisely targeting and isolating shorter cell-free DNA (cfDNA) fragments, the system enriches the fetal fraction in maternal plasma samples, improving sequencing signal-to-noise ratios and reducing uncallable call rates.
The platform enables precise, automated recovery of target cfDNA fractions with an allowable size range from 0 to 20 kb+. Using In-Channel Filter (ICF) cassettes, laboratories can achieve narrow size selection windows (+/-15 bp) tailored specifically to enrich non-normal or fetal cfDNA fragments while rejecting longer background maternal genomic DNA.
LightBench systems deliver market-leading intrinsic target recovery yields between 70% and 80% across size selection protocols, ensuring maximum retention of low-input plasma DNA samples prior to sequencing.
Ranger Technology features dynamic real-time optical tracking and independent electronic field control in each cassette channel. This achieves >97% reproducibility across sample replicates and >95% size selection accuracy, eliminating variability associated with manual liquid handling or bead-based methods.
LightBench platforms integrate three critical functions into one benchtop unit: automated DNA size selection, fragment length analytics (50 bp to 150 kb), and solution-based fluorometric quantification. This 3-in-1 integration replaces up to three separate instruments, reducing equipment footprints, setup costs, and benchtop complexity.
Yes, the LightBench Discover incorporates field inversion technology and specialized agarose slab cassettes to perform quantitative and qualitative fragment length analytics for large DNA up to 150 kb. This capability allows labs to assess genomic DNA integrity before shearing or library construction.
Benchtop LightBench instruments process up to 12 channels per size selection run or up to 48 samples per run for fragment analytics. For high-volume production facilities, the NIMBUS Select workstation expands processing capacity up to 96 samples per run with full walkaway liquid handling automation.
The system features a built-in plate-based fluorescence reader capable of measuring DNA concentrations down to 0.2 ng across 96-well and 384-well formats. The control software automatically merges concentration data with fragment length distribution metrics to calculate exact sample molarity, optimizing library dilution and sample pooling prior to sequencing.