Solutions
Empowering high-throughput genomics with simplified, scalable workflows.
The seqWell portfolio is built on a foundation of "True Multiplexing," utilizing the engineered TnX™ transposase to solve the bottlenecks of traditional NGS library prep. Whether you are sequencing single plasmids or thousands of agricultural samples, our solutions eliminate labor-intensive normalization steps and provide uniform coverage across diverse genomic targets.
Navigating the seqWell Ecosystem: A Versatile NGS Portfolio
The modern genomics landscape requires a toolkit that is as diverse as the applications it supports. The seqWell portfolio is designed to simplify and scale NGS library preparation across a vast spectrum, from rapid screening of synthetic plasmids to high-complexity human whole genome sequencing (WGS) and advanced long-read applications. By leveraging specialized transposase technologies like the engineered TnX™, seqWell kits provide a bridge between the speed of tagmentation and the high data quality of traditional ligation methods.
This overview maps every application detailed in the seqWell guide to the best-fit portfolio match, highlighting where workflows naturally overlap to give researchers maximum flexibility.
Clinical-Grade Precision for the Most Demanding Genomes.
Human & High-Complexity Mammalian Genomics
If you focus on detecting germline variants, SNPs, and complex structural changes in large genomes where uniform coverage and high molecular complexity are non-negotiable.
When working with Human & High-Complexity Mammalian Genomics, MosaiX™ is the best fit because it utilizes an engineered TnX transposase that significantly reduces sequence-dependent insertion bias. This ensures uniform coverage and high molecular complexity, essential for clinical applications like genetic disease diagnosis and personalized medicine, without the "blind spots" found in traditional tagmentation methods.
If you are looking for Long-Read Sequencing (PacBio® HiFi), LongPlex™ is the best fit because it replaces inconsistent mechanical shearing with a streamlined enzymatic workflow. It allows for early pooling of up to 96 samples, drastically reducing the cost and hands-on time required to generate 8–12 kb fragments for de novo assembly or structural variant analysis.
Scaling Sustainability Through High-Throughput Genotyping.
Agricultural & Population Genomics
If you are managing high-volume screening projects where cost-per-sample and workflow automation are your primary drivers, especially for "SKIM-seq" or low-pass sequencing of large populations.
When working with Agricultural & Population Genomics, AgriPrep™ is the best fit because it features integrated auto-normalization that eliminates the need for labor-intensive manual quantification and dilution. This ensures highly consistent read depth across thousands of samples, reducing the need for expensive over-sequencing and allowing you to maximize flow cell capacity for applications like genomic selection, trait mapping, and Genotyping-by-Sequencing (GBS).
If your agricultural research requires high-resolution structural data or chromosomal phasing, LongPlex™ XL (10-12 kb) is the best fit because it provides an enzymatic alternative to mechanical shearing for long-read sequencing. It allows you to generate the long fragments necessary for complex plant and animal genomes while maintaining a streamlined, cost-effective workflow.
Rapid Surveillance from Single Isolates to Complex Communities
Microbial & Infectious Disease Research
If you cover a broad range of biological scales, from single bacterial isolates to complex environmental metagenomic communities where rapid turnaround and reliable representation are critical.
When working with Microbial & Infectious Disease Research, ExpressPlex™ 2.0 is the best fit because they offer an ultra-fast, 90-minute workflow that handles everything from sequence confirmation and construct screening to deep metagenomic profiling.
If your microbial research involves resolving highly repetitive regions or complex genomic structures, LongPlex™ (<10 kb) is the best fit because it enables high-throughput long-read sequencing. It allows you to bypass the bottlenecks of mechanical shearing, providing the long-fragment data necessary to fully assemble microbial genomes and characterize infectious agents with higher resolution.
Precision Long-Reads Without the Pressure of Mechanical Shearing.
Long-Read Sequencing (PacBio® HiFi)
If you need to generate high-quality long fragments (8–10 kb) for applications such as microbial WGS, metagenomics, or low-pass large genome sequencing on a PacBio platform.
When working with Long-Read Sequencing (PacBio® HiFi), LongPlex™ is the best fit because it provides a streamlined enzymatic alternative to the time-consuming and inconsistent process of mechanical shearing with devices like the Covaris or Megaruptor. This technology simultaneously fragments and barcodes DNA, allowing you to pool up to 96 samples into a single SMRTbell® library preparation. This approach drastically reduces both the labor and the overall cost associated with long-read sequencing while ensuring reliable fragment lengths for de novo assembly.
The Fastest Path from Synthetic Construct to Sequence Verification
Plasmids & Amplicons
If you are managing the high-throughput sequencing of synthetic biology "bread and butter" components, such as whole plasmids, PCR products, or targeted amplicons like HLA genotyping.
When working with Plasmids & Amplicons, ExpressPlex™ 2.0 is the best fit because it offers the fastest path from synthetic construct to sequence verification through a one-step, 90-minute workflow. Its integrated auto-normalization allows you to pool samples by volume, bypassing the time-consuming steps of individual quantification and dilution while ensuring high-quality data for construct screening and sequence confirmation.
If your project involves unique payloads, epigenetic assays, or requires universal adapters not found in standard ready-to-use kits, Tagify™ Custom is the best fit. It provides the flexibility to adapt seqWell’s core transposase technology to specialized or proprietary library preparation requirements without sacrificing efficiency.
Validate Your Edits with Absolute Confidence
Gene Editing & Targeted Quality Control
If you are performing specialized assays to verify CRISPR on-target editing and detect unintended off-target structural changes, such as translocations or indels.
When working with Gene Editing & Targeted Quality Control (QC), Tagify™ i5 UMI is the best fit because it is specifically designed for high-sensitivity detection of on- and off-target events. By utilizing Unique Molecular Identifiers (UMIs), it provides the quantitative accuracy needed for rigorous assays like UDiTaS™ or RGen-seq, ensuring that your genomic modifications are precise and predictable.
If your workflow begins with the creation of synthetic editing tools, ExpressPlex™ 2.0 often handles the initial high-throughput screening of gene editing constructs and plasmids. Once the constructs are verified, Tagify™ then performs the deep genomic QC required to confirm the final editing outcome in the host cell line.